Kun:LabNotes/ASE/2007-12-31: Difference between revisions

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(New page: ==Exp.1 Make gDNA-free cDNA from GM20431, GC1EP and Hues6== GM20431(06/04/07) GC1EP(06/14/07) Hues6(05/14/07) total RNA 1.25ug/ul x8ul 645ng/ul x 15ul 2...)
 
Line 5: Line 5:
     H2O (DEPC)        17ul                10ul              0ul
     H2O (DEPC)        17ul                10ul              0ul
     DNase I(2u/ul)    2ul                2ul              2ul
     DNase I(2u/ul)    2ul                2ul              2ul
    RNase Inhibitor  0.5ul              0.5ul            0.5ul
     37C 30min -> 75C 10min -> Cleanup with RNeasy columns
     37C 30min -> 75C 10min -> Cleanup with RNeasy columns

Revision as of 23:01, 31 December 2007

Exp.1 Make gDNA-free cDNA from GM20431, GC1EP and Hues6

                  GM20431(06/04/07)   GC1EP(06/14/07) Hues6(05/14/07)
   total RNA       1.25ug/ul x8ul     645ng/ul x 15ul  270ng/ul x 25ul
   10x buffer         3ul                 3ul               3ul
   H2O (DEPC)        17ul                10ul               0ul
   DNase I(2u/ul)     2ul                 2ul               2ul
   RNase Inhibitor  0.5ul               0.5ul             0.5ul
   37C 30min -> 75C 10min -> Cleanup with RNeasy columns