AlanFung:Protocol/Genome Analyzer: Difference between revisions

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*Position of each reagent in the Genome Analyzer
*Position of each reagent in the Genome Analyzer
  NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER
  NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER
  Position    Reagent                Volume              Container
  '''Position    Reagent                Volume              Container'''
  1          Incorporation Mix      Varies w/ cycle #  50ml amber tube
  1          Incorporation Mix      Varies w/ cycle #  50ml amber tube
  2          DI H2O                                    259ml bottle
  2          DI H2O                                    250ml bottle
  3          Scanning Mix          50ml                50ml tube
  3          Scanning Mix          50ml                50ml tube
  4          High Salt Buffer                          250ml bottle
  4          High Salt Buffer                          250ml bottle

Revision as of 18:22, 25 June 2009

Preparing Reagents for the Genome Analyzer

  • Genome analyzer Reagent Compartment & Reagent Position

File:Reagent Position.jpg

  • Position of each reagent in the Genome Analyzer
NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER
Position    Reagent                Volume              Container
1           Incorporation Mix      Varies w/ cycle #   50ml amber tube
2           DI H2O                                     250ml bottle
3           Scanning Mix           50ml                50ml tube
4           High Salt Buffer                           250ml bottle
5           Incorporation buffer   150ml               250ml bottle
6           Cleavage Mix           50ml                50ml tube
7           Cleavage Buffer        70ml                250ml bottle