Alice:LabNotes/2009-7-6: Difference between revisions

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>Jie deng
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>Jie deng
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Line 1: Line 1:
  Pooled all the captured product based on the following table:
  Pooled all the captured product based on the following table:
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''sample'''
| align="center" style="background:#f0f0f0;"|'''sample'''
| align="center" style="background:#f0f0f0;"|'''a1'''
| align="center" style="background:#f0f0f0;"|'''a1'''
Line 18: Line 18:
| total (ng)||160||80||115.2||116.8||||141||70.5||101.5||103
| total (ng)||160||80||115.2||116.8||||141||70.5||101.5||103
|-
|-
| ||||||||||||||||||
|  
|-
|}
| sample||c1||c2||c3||c4||||d1||d2||d3||d4
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''sample'''
| align="center" style="background:#f0f0f0;"|'''c1'''
| align="center" style="background:#f0f0f0;"|'''c2'''
| align="center" style="background:#f0f0f0;"|'''c3'''
| align="center" style="background:#f0f0f0;"|'''c4'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''d1'''
| align="center" style="background:#f0f0f0;"|'''d2'''
| align="center" style="background:#f0f0f0;"|'''d3'''
| align="center" style="background:#f0f0f0;"|'''d4'''
|-
|-
| concentration (ng/ul)||13||7.2||4.9||5.4||||14||7.8||6.6||7.4
| concentration (ng/ul)||13||7.2||4.9||5.4||||14||7.8||6.6||7.4
Line 28: Line 39:
| total (ng)||130||65||93.6||95||||140||70||100.8||102.2
| total (ng)||130||65||93.6||95||||140||70||100.8||102.2
|-
|-
| ||||||||||||||||||
|  
|-
|}
| sample||e1||e2||e3||e4||||f1||f2||f3||f4
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''sample'''
| align="center" style="background:#f0f0f0;"|'''e1'''
| align="center" style="background:#f0f0f0;"|'''e2'''
| align="center" style="background:#f0f0f0;"|'''e3'''
| align="center" style="background:#f0f0f0;"|'''e4'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''f1'''
| align="center" style="background:#f0f0f0;"|'''f2'''
| align="center" style="background:#f0f0f0;"|'''f3'''
| align="center" style="background:#f0f0f0;"|'''f4'''
|-
|-
| concentration (ng/ul)||11||3.1||3.4||4.7||||9.5||5.5||2.2||4.6
| concentration (ng/ul)||11||3.1||3.4||4.7||||9.5||5.5||2.2||4.6
Line 38: Line 60:
| total (ng)||80.3||40.15||57.8||58.6||||27.5||13.8||19.8||20
| total (ng)||80.3||40.15||57.8||58.6||||27.5||13.8||19.8||20
|-
|-
| ||||||||||||||||||
|  
|-
|}
| sample||DF1||DF2||DF3||DF4||||FS1||FS2||FS3||FS4
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''sample'''
| align="center" style="background:#f0f0f0;"|'''DF1'''
| align="center" style="background:#f0f0f0;"|'''DF2'''
| align="center" style="background:#f0f0f0;"|'''DF3'''
| align="center" style="background:#f0f0f0;"|'''DF4'''
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''FS1'''
| align="center" style="background:#f0f0f0;"|'''FS2'''
| align="center" style="background:#f0f0f0;"|'''FS3'''
| align="center" style="background:#f0f0f0;"|'''FS4'''
|-
|-
| concentration (ng/ul)||17.7||10.1||6.2||11.7||||15.3||4.1||5.4||5.2
| concentration (ng/ul)||17.7||10.1||6.2||11.7||||15.3||4.1||5.4||5.2

Revision as of 01:31, 13 July 2009

Pooled all the captured product based on the following table:
sample a1 a2 a3 a4 ' b1 b2 b3 b4
concentration (ng/ul) 16 9.7 6.7 12.3 9.4 7.6 5.1 9.8
volume (ul) 10 8.2 17 9.5 15 9.3 20 10.5
total (ng) 160 80 115.2 116.8 141 70.5 101.5 103
sample c1 c2 c3 c4 ' d1 d2 d3 d4
concentration (ng/ul) 13 7.2 4.9 5.4 14 7.8 6.6 7.4
volume (ul) 10 9 19 17.5 10 9 15 14
total (ng) 130 65 93.6 95 140 70 100.8 102.2
sample e1 e2 e3 e4 ' f1 f2 f3 f4
concentration (ng/ul) 11 3.1 3.4 4.7 9.5 5.5 2.2 4.6
volume (ul) 7.3 13 17 12.5 2.9 2.5 9 4.4
total (ng) 80.3 40.15 57.8 58.6 27.5 13.8 19.8 20
sample DF1 DF2 DF3 DF4 ' FS1 FS2 FS3 FS4
concentration (ng/ul) 17.7 10.1 6.2 11.7 15.3 4.1 5.4 5.2
volume (ul) 9.2 8.1 19 10.2 8.6 16 18 18.5
total (ng) 163.6 81.8 117.8 119.4 131.8 65.9 95 111.7
Sent out the following tubes for Covaris shearing to Dr. Billy Li (Harvard) 
PGP1_070809: 25ul (13ng/ul)
PGP2_070809: 30ul (9.7ng/ul)
PGP3_070809: 32ul (8.4ng/ul)
NA12878_070809: 26ul (11ng/ul)
PGP5_070809: 31ul (5.5ng/ul)
DF6-9-9_070809: 33ul (5.4ng/ul)
foreskin_070809: 44ul (6.4ng/ul)