Jie:LabNotes/CpgSeq/2009-7-14: Difference between revisions
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[[Image:20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75.jpg]]20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75 | [[Image:20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75.jpg]]20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75 | ||
==Sample Par. ship to Billy== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample ID''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration (ng/ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume (ul)''' | |||
| align="center" style="background:#f0f0f0;"|'''Total Amt. (ng)''' | |||
|- | |||
| Par_1||21.22||30||636.6 | |||
|- | |||
| Par_2||15.25||30||457.5 | |||
|- | |||
| Par_3||18.05||30||541.5 | |||
|- | |||
| Par_4||19.97||30||599.1 | |||
|- | |||
| Par_5||29.63||30||888.9 | |||
|- | |||
| Par_6||15.82||30||474.6 | |||
|- | |||
| Par_7||35.41||30||1062.3 | |||
|- | |||
| Par_8||46.59||30||1397.7 | |||
|- | |||
| Par_9||22.29||30||668.7 | |||
|- | |||
| Par_10||30.4||30||912 | |||
|- | |||
| | |||
|} |
Revision as of 21:57, 14 July 2009
genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75
reaction system x6 H2O 20ul 120ul 2x Mastermix 25ul 150ul AmpF(10uM) 2ul 12ul AmpR(10uM) 2ul 12ul template 1ul/each from each sample Total 50ul 300ul 94C 2min -> (94C 45S -> 55C 45S -> 72C 45S) x 8 ->72C 3 min -> 15C hold.
File:20090714 genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75.jpg20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75
Sample Par. ship to Billy
Sample ID | Concentration (ng/ul) | Volume (ul) | Total Amt. (ng) |
Par_1 | 21.22 | 30 | 636.6 |
Par_2 | 15.25 | 30 | 457.5 |
Par_3 | 18.05 | 30 | 541.5 |
Par_4 | 19.97 | 30 | 599.1 |
Par_5 | 29.63 | 30 | 888.9 |
Par_6 | 15.82 | 30 | 474.6 |
Par_7 | 35.41 | 30 | 1062.3 |
Par_8 | 46.59 | 30 | 1397.7 |
Par_9 | 22.29 | 30 | 668.7 |
Par_10 | 30.4 | 30 | 912 |