Jie:LabNotes/CpgSeq/2009-8-7: Difference between revisions
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===cpature by cpg97k === | ===cpature by cpg97k === | ||
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|align="center" style="background:#f0f0f0;"|'''sample concentration''' | |align="center" style="background:#f0f0f0;"|'''sample concentration''' | ||
| align="center" style="background:#f0f0f0;"|'''10xLigase buffer''' | | align="center" style="background:#f0f0f0;"|'''10xLigase buffer''' | ||
| align="center" style="background:#f0f0f0;"|'''template+cpg97k_A( | | align="center" style="background:#f0f0f0;"|'''template+cpg97k_A(60ng/ul_08/10) vol+suppress oligo+H2O''' | ||
| align="center" style="background:#f0f0f0;"|'''template+cpg97k_B( | | align="center" style="background:#f0f0f0;"|'''template+cpg97k_B(60ng/ul_08/10) vol+suppressor oligo+H2O''' | ||
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|||05192A18 ||148.2ng/ul ||1ul|| | |||05192A18 ||148.2ng/ul ||1ul||3+1.5ul+1ul+3.5ul||3+1.5ul+1ul+3.5ul | ||
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|||05192B09 ||127.8ng/ul ||1ul|| | |||05192B09 ||127.8ng/ul ||1ul||3+1.5ul+1ul+3.5ul||3+1.5ul+1ul+3.5ul | ||
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Revision as of 19:56, 11 August 2009
breast cancer patient peripheral blood DNA sample capture by cpg97k
gDNA extraction from blood
I used the Qiagen FlexiGene DNA kit to exact the DNA from blood. yield: 05192A18: 83.5ng/ul x 100ul; 05192B09: 72.7ng/ul x 100ul
Bisulfite conversion of patient DNA
No | sample | sample concentration | sample volumn | ddH2O | conversion reagents | conversed DNA concentration and volumn |
05192A18 | 83.5ng/ul x 1 tube | 20ul | 0ul | 130ul | 148.2ng/ul x 10ul | |
05192B09 | 72.7ng/ulx 1 tubes | 20ul | 0ul | 130ul | 127.8ng/ul x 10ul |
cpature by cpg97k
}No | sample | sample concentration | 10xLigase buffer | template+cpg97k_A(60ng/ul_08/10) vol+suppress oligo+H2O | template+cpg97k_B(60ng/ul_08/10) vol+suppressor oligo+H2O |
05192A18 | 148.2ng/ul | 1ul | 3+1.5ul+1ul+3.5ul | 3+1.5ul+1ul+3.5ul | |
05192B09 | 127.8ng/ul | 1ul | 3+1.5ul+1ul+3.5ul | 3+1.5ul+1ul+3.5ul |