Athurva Gore/LabNotes/2009-8-24: Difference between revisions
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{{ AGLabEntry|2009-8-21|2009-8-25 }} | {{ AGLabEntry|2009-8-21|2009-8-25 }} | ||
=Probe Sets= | =Probe Sets= | ||
* FLYDUP5279 has finished; all old probesets are done | * '''FLYDUP5279''' has finished; all old probesets are done | ||
* Need to create and send invoice to Zymo today | * Need to create and send invoice to Zymo today | ||
** Also mention that probe generation can be rerun without quality filters? | ** Also mention that probe generation can be rerun without quality filters? |
Revision as of 17:07, 24 August 2009
Probe Sets
- FLYDUP5279 has finished; all old probesets are done
- Need to create and send invoice to Zymo today
- Also mention that probe generation can be rerun without quality filters?
- Need to redo TDMR set and Cpg-SNP set, but with a new probe design...
- Constrain arm+target size to 105 bp
- Then modify P2MIP_v9 to use a 45 bp total arm size
- This will allow library construction to not be necessary
- During capture, MME1 digestion removes 17-18 bp from each side of H1_TARGET_H2
- Should ideally leave us with 75 bp segment remaining, 60 bp of which will be target
- This can be read in the sequencer without library construction, allowing for a much quicker and more efficient experimental procedure