Jie:LabNotes/CpgSeq/2009-9-2: Difference between revisions
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>Sam Chiang (New page: ==ASM validation of imprinted genes== HUES42: 81.1ng/ul HUES63: 36.5ng/ul; hFib2: 16.7ng/ul) |
>Jie deng No edit summary |
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==ASM validation of imprinted genes== | ==ASM validation of imprinted genes== | ||
HUES42: 81.1ng/ul | ===I did the bisulfite conversion of HUES42, HUES63 and hFib2=== | ||
HUES63: 36.5ng/ul; | The concentration after bisulfite conversion using zymo kit: | ||
hFib2: 16.7ng/ul | HUES42: 81.1ng/ul | ||
HUES63: 36.5ng/ul; | |||
hFib2: 16.7ng/ul | |||
===PCR with SLC26A10 and SNRPN primers=== | |||
SLC26A10: HUES42 and hFib2; | |||
SNRPN: HUES63; | |||
===TA cloning of the PCR products from the above three samples=== | |||
I send the plate SLC26A10_ |
Revision as of 16:19, 8 September 2009
ASM validation of imprinted genes
I did the bisulfite conversion of HUES42, HUES63 and hFib2
The concentration after bisulfite conversion using zymo kit: HUES42: 81.1ng/ul HUES63: 36.5ng/ul; hFib2: 16.7ng/ul
PCR with SLC26A10 and SNRPN primers
SLC26A10: HUES42 and hFib2; SNRPN: HUES63;
TA cloning of the PCR products from the above three samples
I send the plate SLC26A10_