Jie:LabNotes/CpgSeq/2009-9-2: Difference between revisions

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>Sam Chiang
(New page: ==ASM validation of imprinted genes== HUES42: 81.1ng/ul HUES63: 36.5ng/ul; hFib2: 16.7ng/ul)
 
>Jie deng
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==ASM validation of imprinted genes==
==ASM validation of imprinted genes==
HUES42: 81.1ng/ul
===I did the bisulfite conversion of HUES42, HUES63 and hFib2===
HUES63: 36.5ng/ul;
  The concentration after bisulfite conversion using zymo kit:
hFib2: 16.7ng/ul
  HUES42: 81.1ng/ul
  HUES63: 36.5ng/ul;
  hFib2: 16.7ng/ul
 
===PCR with SLC26A10 and SNRPN primers===
SLC26A10: HUES42 and hFib2;
SNRPN: HUES63;
 
===TA cloning of the PCR products from the above three samples===
I send the plate SLC26A10_

Revision as of 16:19, 8 September 2009

ASM validation of imprinted genes

I did the bisulfite conversion of HUES42, HUES63 and hFib2

 The concentration after bisulfite conversion using zymo kit:
 HUES42: 81.1ng/ul
 HUES63: 36.5ng/ul;
 hFib2: 16.7ng/ul

PCR with SLC26A10 and SNRPN primers

SLC26A10: HUES42 and hFib2;
SNRPN: HUES63;

TA cloning of the PCR products from the above three samples

I send the plate SLC26A10_