Alice:LabNotes/2009-9-7: Difference between revisions
Jump to navigation
Jump to search
>Zsakura2 |
>Zsakura2 |
||
Line 12: | Line 12: | ||
Qiaquick column purification and elute in 30ul of EB. | Qiaquick column purification and elute in 30ul of EB. | ||
PAGE gel result: | |||
[[Image:ZhangLab_2 2009-09-09 16hr 02minphix.jpg]] | |||
QPCR result: 1.59nM | QPCR result: 1.59nM |
Revision as of 23:41, 14 September 2009
Re-amplification of PhiX control library
PCR: x4 PhiX control DNA(10nM) 0.025ul 0.1ul Syb_FP5(100uM) 0.2ul 0.8ul Syb_RP7(100uM) 0.2ul 0.8ul 2x phusion master mix 50ul 200ul 50x SYBR geen 0.2ul 0.8ul H2O 50ul 200ul 98c 30sec -> (98C 10sec -> 65C 20sec -> 72C 20sec) X 11 cycles -> 72C 3min -> 15C
Qiaquick column purification and elute in 30ul of EB. PAGE gel result: File:ZhangLab 2 2009-09-09 16hr 02minphix.jpg QPCR result: 1.59nM