CES36k18bp: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Jbrubake
>Jbrubake
Line 6: Line 6:
  2x Kapa Master Mix      50ul            50ul
  2x Kapa Master Mix      50ul            50ul
  H2O                    50ul            50ul
  H2O                    50ul            50ul
  AP1V61U (100uM)     0.4ul            0.4ul
  AP1V61U (100uM)       0.4ul            0.4ul
  AP2V6 (100uM)     0.4ul            0.4ul
  AP2V6 (100uM)         0.4ul            0.4ul
  95C, 30sec -> (95C, 3sec ->  60C, 50sec -> plate read) x 25 -> 60C, 2min -> 15C hold
  95C, 30sec -> (95C, 3sec ->  60C, 50sec -> plate read) x 25 -> 60C, 2min -> 15C hold

Revision as of 18:25, 4 November 2009

CES36k18bp (CES) Creation

PCR

                         1x               48x
Template               0.4ul           19.2ul 
2x Kapa Master Mix      50ul             50ul
H2O                     50ul             50ul
AP1V61U (100uM)        0.4ul            0.4ul
AP2V6 (100uM)          0.4ul            0.4ul
95C, 30sec -> (95C, 3sec ->  60C, 50sec -> plate read) x 25 -> 60C, 2min -> 15C hold