AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions

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>Alan6017518
>Alan6017518
Line 10: Line 10:
| Library||1||-||-||-
| Library||1||-||-||-
|
|
| 2X Phusion||50||50||750||825
| 2X Phusion||50||750||825
|-
|-
| AmpF6.3NH2 (100uM)||2||2||3||3.3
| AmpF6.3NH2 (100uM)||2||3||3.3
|-
|-
| AmpR6.3NH2 (100uM)||2||2||3||3.3
| AmpR6.3NH2 (100uM)||2||3||3.3
|-
|-
| 50X SYBR Green I||2||2||3||3.3
| 50X SYBR Green I||2||3||3.3
|-
|-
| dH2O||32||43.75||645||709.5
| dH2O||32||645||709.5
|-
|-
|  
|  

Revision as of 05:35, 23 November 2009

CVF-N

  • Reamplify library and ship it out for shearing
' ' ' x4 X1.1
Library 1 - - - 2X Phusion 50 750 825
AmpF6.3NH2 (100uM) 2 3 3.3
AmpR6.3NH2 (100uM) 2 3 3.3
50X SYBR Green I 2 3 3.3
dH2O 32 645 709.5
  • 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min