AlanFung:LabNotes/Sequencing/2009-11-22: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 (→CVF-N) |
>Alan6017518 (→CVF-N) |
||
Line 10: | Line 10: | ||
| Library||1||-||-||- | | Library||1||-||-||- | ||
| | | | ||
| 2X Phusion | | 2X Phusion||50||750||825 | ||
|- | |- | ||
| AmpF6.3NH2 (100uM) | | AmpF6.3NH2 (100uM)||2||3||3.3 | ||
|- | |- | ||
| AmpR6.3NH2 (100uM) | | AmpR6.3NH2 (100uM)||2||3||3.3 | ||
|- | |- | ||
| 50X SYBR Green I | | 50X SYBR Green I||2||3||3.3 | ||
|- | |- | ||
| dH2O||32 | | dH2O||32||645||709.5 | ||
|- | |- | ||
| | | |
Revision as of 05:35, 23 November 2009
CVF-N
- Reamplify library and ship it out for shearing
' | ' | ' | x4 | X1.1 | |||||
Library | 1 | - | - | - | 2X Phusion | 50 | 750 | 825 | |
AmpF6.3NH2 (100uM) | 2 | 3 | 3.3 | ||||||
AmpR6.3NH2 (100uM) | 2 | 3 | 3.3 | ||||||
50X SYBR Green I | 2 | 3 | 3.3 | ||||||
dH2O | 32 | 645 | 709.5 | ||||||
- 98C 1min->6X(98C 10 sec, 58C 20 sec, 72C 20sec)->72C 5 min