AlanFung:LabNotes/Sequencing/2009-11-23: Difference between revisions
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>Alan6017518 (New page: ==Quantification of New amplified Phi-X and all libraries== *Dilute concentration by 10 fold for Har_2, Har_5 *Dilute New amplified PhiX to 1nM *Keep P3, P5, P7 and CVF_N_D as is {| {{tab...) |
>Alan6017518 |
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{| {{table}} | {| {{table}} | ||
| |||| | | ||||X8||X1.1 | ||
|- | |- | ||
| PhiX Control DNA (10nM)||1.000|||| | | PhiX Control DNA (10nM)||1.000|||| | ||
|- | |- | ||
| Syb_FP5 (100uM)||0.200|| | | Syb_FP5 (100uM)||0.200||1.6||1.76 | ||
|- | |- | ||
| Syb_RP7 (100uM)||0.200|| | | Syb_RP7 (100uM)||0.200||1.6||1.76 | ||
|- | |- | ||
| 2X Phusion||25.000|| | | 2X Phusion||25.000||200||220 | ||
|- | |- | ||
| 50X SYBR Green I||0.200|| | | 50X SYBR Green I||0.200||1.6||1.76 | ||
|- | |- | ||
| H20||24.000|| | | H20||24.000||192||211.2 | ||
|- | |- | ||
| | | | ||
|} | |} |
Revision as of 16:30, 23 November 2009
Quantification of New amplified Phi-X and all libraries
- Dilute concentration by 10 fold for Har_2, Har_5
- Dilute New amplified PhiX to 1nM
- Keep P3, P5, P7 and CVF_N_D as is
Samples | ||||
PhiX-Stock | 8/9 P7 | |||
PhiX-Stock | 8/9 P7 | |||
PhiX(1nM) | Har_2 | |||
PhiX(1nM) | Har_2 | |||
8/9 P3 | Har_5 | |||
8/9 P3 | Har_5 | |||
8/9 P5 | CVF_N_D | |||
8/9 P5 | CVF_N_D | |||
- split every 50ul rxn into 2 wells
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
X8 | X1.1 | ||
PhiX Control DNA (10nM) | 1.000 | ||
Syb_FP5 (100uM) | 0.200 | 1.6 | 1.76 |
Syb_RP7 (100uM) | 0.200 | 1.6 | 1.76 |
2X Phusion | 25.000 | 200 | 220 |
50X SYBR Green I | 0.200 | 1.6 | 1.76 |
H20 | 24.000 | 192 | 211.2 |