Dinh:Protocols/Probes Prep Nov24: Difference between revisions

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m (New page: KAPA SYBR FAST qPCR Kit Master Mix (2x) Universal ideally suited for gene expression analysis low copy gene detection microarray validation gene knockdown validation Notes: protect k...)
 
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  always ensure that the product has been fully thawed and mixed before use
  always ensure that the product has been fully thawed and mixed before use
  do not exceed 25 ul reaction volumes
  do not exceed 25 ul reaction volumes
do not exceed 20ng template DNA per 20 ul reaction volume
  novel enzyme in kit enhances the amplification efficiency of both high GC and AT templates
  novel enzyme in kit enhances the amplification efficiency of both high GC and AT templates
Chromo4 RT Detector does not require ROX Reference dye

Revision as of 22:02, 24 November 2009

KAPA SYBR FAST qPCR Kit Master Mix (2x) Universal

ideally suited for gene expression analysis
low copy gene detection
microarray validation
gene knockdown validation

Notes:

protect kit components from light
always ensure that the product has been fully thawed and mixed before use
do not exceed 25 ul reaction volumes
do not exceed 20ng template DNA per 20 ul reaction volume
novel enzyme in kit enhances the amplification efficiency of both high GC and AT templates
Chromo4 RT Detector does not require ROX Reference dye