Dinh:LAB/Bisulfite Sequencing: Difference between revisions

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* Practice on fibroblast cells culture in suspension [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-1 AllPrep DNA/RNAeasy Kit]
* Practice on fibroblast cells culture in suspension [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-1 AllPrep DNA/RNAeasy Kit]
* Breast Cancer Patient Sample (1-9), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-8 DNeasy Blood & Tissue Kit]
* Breast Cancer Patient Sample (1-9), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-8 DNeasy Blood & Tissue Kit]
* Breast Cancer Patient Sample (10-21), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-5 DNeasy Blood & Tissue Kit (improved protocol)]
* Breast Cancer Patient Sample (10-19), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-5 DNeasy Blood & Tissue Kit (improved protocol)]


=Bisulfite Conversion and Capturing=
=Bisulfite Conversion and Capturing=

Revision as of 02:53, 6 January 2010

Probes Preparation

  • Dec 30 Probes Set 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).

DNA Extraction

Bisulfite Conversion and Capturing

  • Improving capturing efficiency of CpG30K set for pipelining, Jan 4 2010 Capture Reaction (2pm),

Notebook

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2010/07/01 view=oneyear </calendar>

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2009/07/01 view=oneyear </calendar>