Dinh:LAB/Bisulfite Sequencing: Difference between revisions

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* Breast Cancer Patient Sample (1-9), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-8 DNeasy Blood & Tissue Kit]
* Breast Cancer Patient Sample (1-9), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-8 DNeasy Blood & Tissue Kit]
* Breast Cancer Patient Sample (10-19), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-5 DNeasy Blood & Tissue Kit (improved protocol)]
* Breast Cancer Patient Sample (10-19), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-5 DNeasy Blood & Tissue Kit (improved protocol)]
* Breast Cancer Patient Sample (1-11, 20-28), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-10 DNeasy Blood & Tissue Kit]


=Bisulfite Conversion and Capturing=
=Bisulfite Conversion and Capturing=
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* Breast Cancer patients (10 samples), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-6 Jan 6 2010] Bisulfite Conversion, [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-7#RT_PCR_of_Bisulfite_Converted_DNA Jan 7 2010] qPCR
* Breast Cancer patients (10 samples), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-6 Jan 6 2010] Bisulfite Conversion, [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-7#RT_PCR_of_Bisulfite_Converted_DNA Jan 7 2010] qPCR
* Breast Cancer patients (1-11, 20-28), [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-12 Jan 12 2010] Bisulfite Conversion, [http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2010-1-13 qPCR_of_Bisulfite_Converted_DNA Jan 13 2010]
* Breast Cancer Patients (1-28) Samples Track, [[Dinh:Breast_Cancer_Methylation]]


=Notebook=
=Notebook=

Revision as of 18:49, 14 January 2010

Probes Preparation

  • Dec 30 Probes Set 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).

DNA Extraction

Bisulfite Conversion and Capturing

  • Improving capturing efficiency of CpG30K set for pipelining, Jan 4 2010 Capture Reaction (2pm), Jan 7 2010 Results.

Notebook

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2010/07/01 view=oneyear </calendar>

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2009/07/01 view=oneyear </calendar>