Dinh:LAB/Bisulfite Sequencing: Difference between revisions

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>Dinh
>Dinh
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=Probes Preparation=
=Probes Preparation=
==CpG30K==
* [[Dinh:Protocols/Probes_Prep_Sep28|Sep 28 2009 Practice Probes Preparation]], CpG30K, 24rxns, Nanodrop: 4.64 ug, End Point Gel Quantification: 1.91 ug
* [[Dinh:Protocols/Probes_Prep_Sep28|Sep 28 2009 Practice Probes Preparation]], CpG30K, 24rxns, Nanodrop: 4.64 ug, End Point Gel Quantification: 1.91 ug
* [[Dinh:Protocols/Probes_Prep_Oct1| Oct 1 2009 Probes Preparation]], CpG30K, 24rxns, Nanodrop:, End Point Gel Quantification: Problem with 2D gel
* [[Dinh:Protocols/Probes_Prep_Oct1| Oct 1 2009 Probes Preparation]], CpG30K, 24rxns, Nanodrop:, End Point Gel Quantification: Problem with 2D gel
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*[http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-29 Dec 30 CpG30K Probes Set] 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).
*[http://genome-tech.ucsd.edu/LabNotes/index.php/Dinh/NOTES/2009-12-29 Dec 30 CpG30K Probes Set] 30ng/ul x 80 ul, purified from 3 plates amplified using EconoTaq (20cycles).
==CpG97K==


=DNA Extraction=
=DNA Extraction=

Revision as of 07:11, 25 January 2010

Probes Preparation

CpG30K

CpG97K

DNA Extraction

Bisulfite Conversion and Capturing

  • Improving capturing efficiency of CpG30K set for pipelining, Jan 4 2010 Capture Reaction (2pm), Jan 7 2010 Results.

Breast Cancer Patients, Diet and Methylation Study

  • Capturing Breast Cancer Patients (samples 1-9), Dec 9 2009 Capture Reaction, Dec 15 2009 E-Gel Size selection, Amplification with iProof
Breast Cancer Patients (1-28) Samples Track, Dinh:Breast_Cancer_Methylation
  • Capturing Test, CpG97A/B. Templates: bis_Jurkat, bis_16. Conditions: 55C vs 60C, 0 Cycling. Jan 10 2010
  • Capture, CpG97A/B. Templates: bis_10-14, bis_Jurkat. Condition: 55C, 0 Cycling. Jan 15 2010
  • Capture Re-test for Sanger Sequencing, CpG97A/B. Template: bis_15. Condition: 55C vs 60C, 0 Cycling. Jan 21 2010

Notebook

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2010/07/01 view=oneyear </calendar>

<calendar> name=Dinh format=%name/NOTES/%year-%month-%day date=2009/07/01 view=oneyear </calendar>