Dinh/NOTES/2009-12-4: Difference between revisions
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>Dinh m (New page: =Quant-iT(TM) OliGreen ssDNA Assay Kit= ==Materials== #Quan-iT OliGreen ssDNA Assay Kit (cat. # 011492, containing: *Quant-iT OliGreen ssDNA reagent, solution in DMSO *20xTE (200 mM Tri...) |
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Line 1: | Line 1: | ||
=Quant-iT(TM) | =Quant-iT(TM) ssDNA Assay Kit= | ||
==Materials== | ==Materials== | ||
#Quan-iT | #Quan-iT ssDNA Assay Kit (cat. # Q32851, containing: | ||
*Quant-iT | *Quant-iT ssDNA reagent, solution in DMSO | ||
*20xTE (200 mM Tris HCl, 20mM EDTA, pH7.5) | *20xTE (200 mM Tris HCl, 20mM EDTA, pH7.5) | ||
*Oligonucleotide standard (100ug/ml = 100ng/ul) | *Oligonucleotide standard (100ug/ml = 100ng/ul) | ||
Line 11: | Line 11: | ||
Table 1. High range standard curve | Table 1. High range standard curve | ||
{| border="1" | {| border="1" | ||
! | |||
!Volume (ul) 1xTE | !Volume (ul) 1xTE | ||
!Volume (ul) of 2ug/ml Oligomer stock | !Volume (ul) of 2ug/ml Oligomer stock | ||
!Volume (ul) of diluted | !Volume (ul) of diluted Working Solution | ||
!Final Oligomer Conc. | !Final Oligomer Conc. | ||
|- | |- | ||
|Standard #1 | |||
|0 | |0 | ||
|250 | |250 | ||
Line 21: | Line 23: | ||
|1ug/ml | |1ug/ml | ||
|- | |- | ||
|Standard #2 | |||
|125 | |125 | ||
|125 | |125 | ||
Line 26: | Line 29: | ||
|500ng/ml | |500ng/ml | ||
|- | |- | ||
|Standard #3 | |||
|225 | |225 | ||
|25 | |25 | ||
Line 31: | Line 35: | ||
|100ng/ml | |100ng/ml | ||
|- | |- | ||
|Standard #4 | |||
|247.5 | |247.5 | ||
|2.5 | |2.5 | ||
Line 36: | Line 41: | ||
|10ng/ml | |10ng/ml | ||
|- | |- | ||
|Standard #5 | |||
|250 | |250 | ||
|0 | |0 |
Revision as of 23:46, 4 December 2009
Quant-iT(TM) ssDNA Assay Kit
Materials
- Quan-iT ssDNA Assay Kit (cat. # Q32851, containing:
*Quant-iT ssDNA reagent, solution in DMSO *20xTE (200 mM Tris HCl, 20mM EDTA, pH7.5) *Oligonucleotide standard (100ug/ml = 100ng/ul)
- Quan-iT OliGreen Assay tubes (500ul)
- Sterile, distilled, DNase free water
Table 1. High range standard curve
Volume (ul) 1xTE | Volume (ul) of 2ug/ml Oligomer stock | Volume (ul) of diluted Working Solution | Final Oligomer Conc. | |
---|---|---|---|---|
Standard #1 | 0 | 250 | 250 | 1ug/ml |
Standard #2 | 125 | 125 | 250 | 500ng/ml |
Standard #3 | 225 | 25 | 250 | 100ng/ml |
Standard #4 | 247.5 | 2.5 | 250 | 10ng/ml |
Standard #5 | 250 | 0 | 250 | blank |
Protocol
1. Preparations
a. Prepare 1xTE buffer (1 ml per each sample to be assayed) b. Prepare working solution by 200 fold dilution of OliGreen ssDNA reagent (in DMSO). For best results, use working solution within few hours of preparation. c. Dilute oligonucleotide standard 50 fold to 2ug/ml