AlanFung:LabNotes/Sequencing/2009-12-5: Difference between revisions

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>Alan6017518
(New page: ==qPCR Quantification== ==PGP4 PGP6 PGP8 PGP9 PGP8D PGP8E Dpn-CV-iF2 Dpn-CV-F2==)
 
>Alan6017518
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==qPCR Quantification==
==qPCR Quantification==
==PGP4 PGP6 PGP8 PGP9 PGP8D PGP8E Dpn-CV-iF2 Dpn-CV-F2==
==PGP4 PGP6 PGP8 PGP9 PGP8D PGP8E Dpn-CV-iF2 Dpn-CV-F2==
{| {{table}}
| Setup||||||||
|-
| Amp Phi-X (1nM)||PGP6||PGP9||PGP8E||Dpn-CV-iF2 (1/10)
|-
| Amp Phi-X (1nM)||PGP6||PGP9||PGP8E||Dpn-CV-iF2 (1/10)
|-
| Amp Phi-X (1nM)||PGP6||PGP9||PGP8E||Dpn-CV-iF2 (1/10)
|-
| Amp Phi-X (1nM)||PGP6||PGP9||PGP8E||Dpn-CV-iF2 (1/10)
|-
| PGP4||PGP8||PGP8D||Dpn-CV-F2 (1/10)||
|-
| PGP4||PGP8||PGP8D||Dpn-CV-F2 (1/10)||
|-
| PGP4||PGP8||PGP8D||Dpn-CV-F2 (1/10)||
|-
| PGP4||PGP8||PGP8D||Dpn-CV-F2 (1/10)||
|-
|
|}
{| {{table}}
| ||||x18||*1.1
|-
| DNA||2||||
|-
| Syb_FP5 (100uM)||0.2||3.6||3.96
|-
| Syb_RP7 (100uM)||0.2||3.6||3.96
|-
| 2X Phusion||25||450||495
|-
| 50X SYBR Green I||0.2||3.6||3.96
|-
| H20||24||432||475.2
|-
| Total||||||982.08
|-
|
|}

Revision as of 18:38, 5 December 2009

qPCR Quantification

PGP4 PGP6 PGP8 PGP9 PGP8D PGP8E Dpn-CV-iF2 Dpn-CV-F2

Setup
Amp Phi-X (1nM) PGP6 PGP9 PGP8E Dpn-CV-iF2 (1/10)
Amp Phi-X (1nM) PGP6 PGP9 PGP8E Dpn-CV-iF2 (1/10)
Amp Phi-X (1nM) PGP6 PGP9 PGP8E Dpn-CV-iF2 (1/10)
Amp Phi-X (1nM) PGP6 PGP9 PGP8E Dpn-CV-iF2 (1/10)
PGP4 PGP8 PGP8D Dpn-CV-F2 (1/10)
PGP4 PGP8 PGP8D Dpn-CV-F2 (1/10)
PGP4 PGP8 PGP8D Dpn-CV-F2 (1/10)
PGP4 PGP8 PGP8D Dpn-CV-F2 (1/10)
x18 *1.1
DNA 2
Syb_FP5 (100uM) 0.2 3.6 3.96
Syb_RP7 (100uM) 0.2 3.6 3.96
2X Phusion 25 450 495
50X SYBR Green I 0.2 3.6 3.96
H20 24 432 475.2
Total 982.08