AlanFung:LabNotes/Sequencing/2009-12-5: Difference between revisions
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>Alan6017518 (New page: ==qPCR Quantification== ==PGP4 PGP6 PGP8 PGP9 PGP8D PGP8E Dpn-CV-iF2 Dpn-CV-F2==) |
>Alan6017518 |
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==qPCR Quantification== | ==qPCR Quantification== | ||
==PGP4 PGP6 PGP8 PGP9 PGP8D PGP8E Dpn-CV-iF2 Dpn-CV-F2== | ==PGP4 PGP6 PGP8 PGP9 PGP8D PGP8E Dpn-CV-iF2 Dpn-CV-F2== | ||
{| {{table}} | |||
| Setup|||||||| | |||
|- | |||
| Amp Phi-X (1nM)||PGP6||PGP9||PGP8E||Dpn-CV-iF2 (1/10) | |||
|- | |||
| Amp Phi-X (1nM)||PGP6||PGP9||PGP8E||Dpn-CV-iF2 (1/10) | |||
|- | |||
| Amp Phi-X (1nM)||PGP6||PGP9||PGP8E||Dpn-CV-iF2 (1/10) | |||
|- | |||
| Amp Phi-X (1nM)||PGP6||PGP9||PGP8E||Dpn-CV-iF2 (1/10) | |||
|- | |||
| PGP4||PGP8||PGP8D||Dpn-CV-F2 (1/10)|| | |||
|- | |||
| PGP4||PGP8||PGP8D||Dpn-CV-F2 (1/10)|| | |||
|- | |||
| PGP4||PGP8||PGP8D||Dpn-CV-F2 (1/10)|| | |||
|- | |||
| PGP4||PGP8||PGP8D||Dpn-CV-F2 (1/10)|| | |||
|- | |||
| | |||
|} | |||
{| {{table}} | |||
| ||||x18||*1.1 | |||
|- | |||
| DNA||2|||| | |||
|- | |||
| Syb_FP5 (100uM)||0.2||3.6||3.96 | |||
|- | |||
| Syb_RP7 (100uM)||0.2||3.6||3.96 | |||
|- | |||
| 2X Phusion||25||450||495 | |||
|- | |||
| 50X SYBR Green I||0.2||3.6||3.96 | |||
|- | |||
| H20||24||432||475.2 | |||
|- | |||
| Total||||||982.08 | |||
|- | |||
| | |||
|} |
Revision as of 18:38, 5 December 2009
qPCR Quantification
PGP4 PGP6 PGP8 PGP9 PGP8D PGP8E Dpn-CV-iF2 Dpn-CV-F2
Setup | ||||
Amp Phi-X (1nM) | PGP6 | PGP9 | PGP8E | Dpn-CV-iF2 (1/10) |
Amp Phi-X (1nM) | PGP6 | PGP9 | PGP8E | Dpn-CV-iF2 (1/10) |
Amp Phi-X (1nM) | PGP6 | PGP9 | PGP8E | Dpn-CV-iF2 (1/10) |
Amp Phi-X (1nM) | PGP6 | PGP9 | PGP8E | Dpn-CV-iF2 (1/10) |
PGP4 | PGP8 | PGP8D | Dpn-CV-F2 (1/10) | |
PGP4 | PGP8 | PGP8D | Dpn-CV-F2 (1/10) | |
PGP4 | PGP8 | PGP8D | Dpn-CV-F2 (1/10) | |
PGP4 | PGP8 | PGP8D | Dpn-CV-F2 (1/10) | |
x18 | *1.1 | ||
DNA | 2 | ||
Syb_FP5 (100uM) | 0.2 | 3.6 | 3.96 |
Syb_RP7 (100uM) | 0.2 | 3.6 | 3.96 |
2X Phusion | 25 | 450 | 495 |
50X SYBR Green I | 0.2 | 3.6 | 3.96 |
H20 | 24 | 432 | 475.2 |
Total | 982.08 | ||