Illumina GA/Oligo info: Difference between revisions

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| align="center" style="background:#f0f0f0;"|'''Reference'''
| align="center" style="background:#f0f0f0;"|'''Reference'''
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| Right Probe Linker, No barcode (forward) || AmpF6.3Sol || ||None||None
| Left Probe Linker, Barcode 1: CGTGAT || AmpR6.3Ind1 || ||None||None
| Left Probe Linker, Barcode 1: CGTGAT || AmpR6.3Ind1 || ||None||None
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Revision as of 21:26, 15 July 2010

Name in original paper Nick-name Sequence (5\'->3\') Sequence Modification Reference
A_adapter_t Long Y-adaptor (top) AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T 3'-Phosphorothioate bond [1]
A_adapter_b Long Y-adaptor (bottom) GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTG None [1]
PE_t_adapter Regular Y-adaptor (top) ACACTCTTTCCCTACACGACGCTCTTCCGATC*T 3'-Phosphorothioate bond [2]
PE_b_adapter Regular Y-adaptor (bottom) /5Phos/GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG 5'-phosphorylation [2]
PCR_F Sol. Amp. (Forward) AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T 3'-Phosphorothioate bond [2]
PCR_R Sol. Amp. (Reverse) CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC*T 3'-Phosphorothioate bond [2]
Syb_FP5 Sol. Quant. (Forward) ATGATACGGCGACCACCGAG None [2]
Syb_RP7 Sol. Quant. (Reverse) CAAGCAGAAGACGGCATACGAG None [2]
PE_t_N2 Y-adaptor for MmeI ligation (top) ACACTCTTTCCCTACACGACGCTCTTCCGATCTN*N 3'-Phosphorothioate bond
PE_N2 Y-adaptor for MmeI ligation (top) ACACTCTTTCCCTACACGACGCTCTTCCGATC*NN 3'-Phosphorothioate bond
PE_b_A Y-adaptor for MmeI ligation (bottom) /5Phos/AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG 5'-phosphorylation
PE_t_Met bis-seq(Forward) A/iMe-dC/A/iMe-dC/T/iMe-dC/TTT/iMe-dC//iMe-dC//iMe-dC/TA/iMe-dC/ACGACG/iMe-dC/T/iMe-dC/TT/iMe-dC/CGAT/iMe-dC/*T 3-phosphorothiate, 5'-Methyl
PE_b_Met bis-seq (Reverse) /5Phos/GATCGGAAGAGCGGTT/iMe-dC/AG/iMe-dC/AGGAATG/iMe-dC/CGAG 5'-phosphorylated, 5'-Methyl
RC_PE_t_adapter RC_Regular Y-adaptor with Biotin (top) /5Biosg/TTTTTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT 5'-Biotin
RC_PE_b_adapter RC_Regular Y-adaptor with Biotin (bottom) /5Biosg/TTTTTTTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC 5'-Biotin
Small RNA Sequencing Primer Solexa-TSS-Seq CGACAGGTTCAGAGTTCTACAGTCCGACGATC

Multiplexing Primers

Name Nick-name Sequence (5\'->3\') Sequence Modification Reference
Right Probe Linker, No barcode (forward) AmpF6.3Sol None None Left Probe Linker, Barcode 1: CGTGAT AmpR6.3Ind1 None None
Left Probe Linker, Barcode 2: ACATCG AmpR6.3Ind2 None None
Left Probe Linker, Barcode 3: GCCTAA AmpR6.3Ind3 None None
Left Probe Linker, Barcode 4: TGGTCA AmpR6.3Ind4 None None
Left Probe Linker, Barcode 5: CACTGT AmpR6.3Ind5 None None
Left Probe Linker, Barcode 6: ATTGGC AmpR6.3Ind6 None None
Left Probe Linker, Barcode 7: GATCTG AmpR6.3Ind7 None None
Left Probe Linker, Barcode 8: TCAAGT AmpR6.3Ind8 None None
Left Probe Linker, Barcode 9: CTGATC AmpR6.3Ind9 None None
Left Probe Linker, Barcode 10: AAGCTA AmpR6.3Ind10 None None
Left Probe Linker, Barcode 11: GTAGCC AmpR6.3Ind11 None None
Left Probe Linker, Barcode 12: TACAAG AmpR6.3Ind12 None None