AlanFung:LabNotes/CTCF/2010-1-15: Difference between revisions
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==PCR quantification of Bisulfite treated samples using 3 paris of CHR primers== | ==PCR quantification of Bisulfite treated samples using 3 paris of CHR bilsulfite conversion primers== | ||
==On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP== | ==On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP== |
Revision as of 16:28, 15 January 2010
Quantify Bisulfite Converted Samples with Qubit ssDNA Kit
On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP
7 samples Prepare 7 Qubit Assay tubes Prepare 7*200ul diluted dye=1400ul Mix 7ul dye with 1393ul buffer
Mix 2ul samples with 98ul diluted dye vortex spin down and wait for 2 mins Result