Protease and Proteinase Trials: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Jbrubake
>Jbrubake
Line 13: Line 13:


2 columns/strips of sorted chromosomes were collected from Sam. The strips were separated as follows:
2 columns/strips of sorted chromosomes were collected from Sam. The strips were separated as follows:
                | H |  - Head
            | H |  - Head
  Row 1    | o | - No chromosomes
  Row 1    | o | - No chromosomes
  Row 2    | o | - 1 chromosome
  Row 2    | o | - 1 chromosome
Line 22: Line 22:
  Row 7    | o | - 23 chromosomes
  Row 7    | o | - 23 chromosomes
  Row 8    | o | - 23 chromosomes
  Row 8    | o | - 23 chromosomes
                | A | - Tail
            | A | - Tail

Revision as of 22:29, 7 April 2010

Two different denaturants were used:

  • Protease (Qiagen) - abbreviated P.
  • Proteinase K (Invitrogen) - abbreviated P.K.

Solution Preparations

The protease (Qiagen) was easily reconstituted: 7uL water per vial

See Proteinase K Protocol for preparation of Proteinase K (Invitrogen)

3/31/10

2 columns/strips of sorted chromosomes were collected from Sam. The strips were separated as follows:

           | H |  - Head
Row 1     | o | - No chromosomes
Row 2     | o | - 1 chromosome
Row 3     | o | - 12 chromosomes
Row 4     | o | - 12 chromosomes
Row 5     | o | - 12 chromosomes
Row 6     | o | - 23 chromosomes
Row 7     | o | - 23 chromosomes
Row 8     | o | - 23 chromosomes
           | A | - Tail