PrepGEM (ZyGEM) Preparation: Difference between revisions

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>Jbrubake
(Created page with '=Preparation= Preparing the PrepGEM denaturant is very simple: Per reaction: - 1uL 10X Gold Buffer - 0.9uL water - 0.1uL PrepGEM substrate')
 
>Jbrubake
 
Line 7: Line 7:
  - 0.9uL water
  - 0.9uL water
  - 0.1uL PrepGEM substrate
  - 0.1uL PrepGEM substrate
---Revised (as of 4/30/10)---
Per reaction:
- 0.1uL 10X Gold Buffer
- 0.89uL water
- 0.01uL PrepGEM substrate
'''Note:''' In order to make a reasonable mixture, enough volume for at least 10 reactions should be mixed at once and split up as much as needed.

Latest revision as of 20:52, 3 May 2010

Preparation[edit]

Preparing the PrepGEM denaturant is very simple:

Per reaction:
- 1uL 10X Gold Buffer
- 0.9uL water
- 0.1uL PrepGEM substrate

---Revised (as of 4/30/10)---

Per reaction:
- 0.1uL 10X Gold Buffer
- 0.89uL water
- 0.01uL PrepGEM substrate


Note: In order to make a reasonable mixture, enough volume for at least 10 reactions should be mixed at once and split up as much as needed.