Noi:Probe preparation data/Probes Prep: Difference between revisions
Jump to navigation
Jump to search
>Noi (Created page with '=Summary of DMR220k probe set production= {| {{table}} border = 1 | align="center" style="background:#f0f0f0;"|'''Probe set''' | align="center" style="background:#f0f0f0;"|'''No…') |
>Noi No edit summary |
||
Line 11: | Line 11: | ||
| align="center" style="background:#f0f0f0;"|'''for DNA capture''' | | align="center" style="background:#f0f0f0;"|'''for DNA capture''' | ||
|- | |- | ||
| S1||96 rxn||2010_10_24||Kapa||26. | | S1||96 rxn||2010_10_24||Kapa||26.20||250.00||6.50||diluted to 2ng/ul vol 100ul | ||
|- | |- | ||
| S2||96 rxn||2010_10_24||Kapa||30. | | S2||96 rxn||2010_10_24||Kapa||30.10||250.00||7.50||diluted to 2ng/ul vol 100ul | ||
|- | |- | ||
| S3||96 rxn||2010_10_24||Kapa||23. | | S3||96 rxn||2010_10_24||Kapa||23.20||250.00||5.80|| | ||
|- | |- | ||
| S4||96 rxn||2010_10_21||Kapa||24. | | S4||96 rxn||2010_10_21||Kapa||24.80||200.00||4.90|| | ||
|- | |- | ||
| S5||96 rxn||2010_10_21||Kapa||26. | | S5||96 rxn||2010_10_21||Kapa||26.70||200.00||5.30|| | ||
|- | |- | ||
| S6||96 rxn||2010_10_22||Kapa||27. | | S6||96 rxn||2010_10_22||Kapa||27.70||200.00||5.50|| | ||
|- | |- | ||
| S7||96 rxn||2010_10_22||Kapa||32||200||6. | | S7||96 rxn||2010_10_22||Kapa||32.00||200.00||6.40|| | ||
|- | |- | ||
| | | | ||
Line 29: | Line 29: | ||
| 1st prep||||||||||||||Total (ug) | | 1st prep||||||||||||||Total (ug) | ||
|- | |- | ||
| S6||96 rxn||2010_10_01||EconoTaq||24. | | S6||96 rxn||2010_10_01||EconoTaq||24.60||102.00||2.50||8.00 | ||
|- | |- | ||
| S7||96 rxn||2010_09_27||Kapa||36. | | S7||96 rxn||2010_09_27||Kapa||36.90||200.00||7.30||13.70 | ||
|}<br> | |||
{| {{table}} border = 1 | |||
| align="center" style="background:#f0f0f0;"|'''Probe set''' | |||
| align="center" style="background:#f0f0f0;"|'''amount used in PAGE SS (ug)''' | |||
| align="center" style="background:#f0f0f0;"|'''yields (ug)''' | |||
| align="center" style="background:#f0f0f0;"|'''%yields''' | |||
| align="center" style="background:#f0f0f0;"|'''% digestion''' | |||
| align="center" style="background:#f0f0f0;"|'''total expected complete digestion yields''' | |||
| align="center" style="background:#f0f0f0;"|'''%recovery''' | |||
|- | |||
| S1||13.00||6.50||50.00||92.00||11.96||54.35 | |||
|- | |||
| S2||15.60||7.50||48.08||92.00||14.35||52.26 | |||
|- | |||
| S3||13.80||5.80||42.03||94.00||12.97||44.71 | |||
|- | |||
| S4||10.70||4.90||45.79||93.00||9.95||49.24 | |||
|- | |||
| S5||9.60||5.30||55.21||92.00||8.83||60.01 | |||
|- | |||
| S6||12.00||5.50||45.83||>90||10.80||50.93 | |||
|- | |||
| S7||12.40||6.40||51.61||93.00||11.53||55.50 | |||
|} | |} |
Revision as of 00:54, 9 December 2010
Summary of DMR220k probe set production
Probe set | No. of rxn | Date of production | Enzyme | Conc. (ng/ul) | Vol. (ul) | Yields (ug) | for DNA capture |
S1 | 96 rxn | 2010_10_24 | Kapa | 26.20 | 250.00 | 6.50 | diluted to 2ng/ul vol 100ul |
S2 | 96 rxn | 2010_10_24 | Kapa | 30.10 | 250.00 | 7.50 | diluted to 2ng/ul vol 100ul |
S3 | 96 rxn | 2010_10_24 | Kapa | 23.20 | 250.00 | 5.80 | |
S4 | 96 rxn | 2010_10_21 | Kapa | 24.80 | 200.00 | 4.90 | |
S5 | 96 rxn | 2010_10_21 | Kapa | 26.70 | 200.00 | 5.30 | |
S6 | 96 rxn | 2010_10_22 | Kapa | 27.70 | 200.00 | 5.50 | |
S7 | 96 rxn | 2010_10_22 | Kapa | 32.00 | 200.00 | 6.40 | |
1st prep | Total (ug) | ||||||
S6 | 96 rxn | 2010_10_01 | EconoTaq | 24.60 | 102.00 | 2.50 | 8.00 |
S7 | 96 rxn | 2010_09_27 | Kapa | 36.90 | 200.00 | 7.30 | 13.70 |
Probe set | amount used in PAGE SS (ug) | yields (ug) | %yields | % digestion | total expected complete digestion yields | %recovery |
S1 | 13.00 | 6.50 | 50.00 | 92.00 | 11.96 | 54.35 |
S2 | 15.60 | 7.50 | 48.08 | 92.00 | 14.35 | 52.26 |
S3 | 13.80 | 5.80 | 42.03 | 94.00 | 12.97 | 44.71 |
S4 | 10.70 | 4.90 | 45.79 | 93.00 | 9.95 | 49.24 |
S5 | 9.60 | 5.30 | 55.21 | 92.00 | 8.83 | 60.01 |
S6 | 12.00 | 5.50 | 45.83 | >90 | 10.80 | 50.93 |
S7 | 12.40 | 6.40 | 51.61 | 93.00 | 11.53 | 55.50 |