Rui:SingelCell: Difference between revisions

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==Protocols==
==Protocols==
===Reverse transcription===
===PCR-based protocol===
===IVT-based protocol===
===Circulation-based protocol===
 
==2011==
==2011==
<calendar>
<calendar>

Revision as of 19:41, 9 January 2011

Background

  • Goal: the smallest functional unit; the exact quantity; all full-length RNAs; all classes; at single-base resolution
  • Step 1: single cell; exact quantity; the 3' end; mRNA; RNAseq

Protocols

PCR-based protocol

IVT-based protocol

Circulation-based protocol

2011

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>