Rui:LabNotes/SingleCell/2011-1-20: Difference between revisions
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>RuiLiu (Created page with '1. Measure the concentration of the RNA samples *Hues 54 414.50ng/ul 2.06 2.01 *Hues6-ES 641.13ng/ul 2.12 2.11 *Hues6-EB 561.99ng/ul 2.11 2.11 2. Examine the int…') |
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1. Measure the concentration of the RNA samples | =1. Measure the concentration of the RNA samples= | ||
-------------------------------------------------------------- | |||
RNA ID [ng/ul] 260/280 260/230 | |||
-------------------------------------------------------------- | |||
Hues54 414.50 2.06 2.01 | |||
Hues6-ES 641.13 2.12 2.11 | |||
Hues6-EB 561.99 2.11 2.11 | |||
-------------------------------------------------------------- | |||
2. Examine the integrity of the RNA samples | =2. Examine the integrity of the RNA samples= | ||
* 100ml agarose gel + 8ul SYBR safe | * 100ml agarose gel + 8ul SYBR safe | ||
3. Dilute RNA samples in PCR 8-tube strip (-80C) | =3. Dilute RNA samples in PCR 8-tube strip (-80C)= | ||
1) 1:10 | |||
2) 1:10 | |||
3) 1:4/1:6/1:5.6 --> 1ng/ul | |||
4) 1:5 --> 0.2ng/ul | |||
5) 1:10 --> 0.02ng/ul |
Revision as of 20:58, 20 January 2011
1. Measure the concentration of the RNA samples
-------------------------------------------------------------- RNA ID [ng/ul] 260/280 260/230 -------------------------------------------------------------- Hues54 414.50 2.06 2.01 Hues6-ES 641.13 2.12 2.11 Hues6-EB 561.99 2.11 2.11 --------------------------------------------------------------
2. Examine the integrity of the RNA samples
- 100ml agarose gel + 8ul SYBR safe
3. Dilute RNA samples in PCR 8-tube strip (-80C)
1) 1:10 2) 1:10 3) 1:4/1:6/1:5.6 --> 1ng/ul 4) 1:5 --> 0.2ng/ul 5) 1:10 --> 0.02ng/ul