Rui:LabNotes/SingleCell/2011-1-20: Difference between revisions

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>RuiLiu
(Created page with '1. Measure the concentration of the RNA samples *Hues 54 414.50ng/ul 2.06 2.01 *Hues6-ES 641.13ng/ul 2.12 2.11 *Hues6-EB 561.99ng/ul 2.11 2.11 2. Examine the int…')
 
>RuiLiu
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1. Measure the concentration of the RNA samples
=1. Measure the concentration of the RNA samples=
*Hues 54  414.50ng/ul  2.06   2.01
--------------------------------------------------------------
*Hues6-ES  641.13ng/ul  2.12   2.11
RNA ID [ng/ul] 260/280 260/230
*Hues6-EB  561.99ng/ul  2.11   2.11
--------------------------------------------------------------
Hues54 414.50         2.06         2.01
Hues6-ES 641.13         2.12         2.11
Hues6-EB 561.99         2.11         2.11
--------------------------------------------------------------


2. Examine the integrity of the RNA samples
=2. Examine the integrity of the RNA samples=
* 100ml agarose gel + 8ul SYBR safe
* 100ml agarose gel + 8ul SYBR safe


3. Dilute RNA samples in PCR 8-tube strip (-80C)
=3. Dilute RNA samples in PCR 8-tube strip (-80C)=
*1) 1:10
1) 1:10
*2) 1:10
2) 1:10
*3) 1:4/1:6/1:5.6 --> 1ng/ul
3) 1:4/1:6/1:5.6 --> 1ng/ul
*4) 1:5 --> 0.2ng/ul
4) 1:5 --> 0.2ng/ul
*5) 1:10 --> 0.02ng/ul
5) 1:10 --> 0.02ng/ul

Revision as of 20:58, 20 January 2011

1. Measure the concentration of the RNA samples

--------------------------------------------------------------
RNA ID 	 [ng/ul]	260/280 	260/230
-------------------------------------------------------------- 
Hues54 	 414.50	        2.06	        2.01
Hues6-ES	 641.13	        2.12	        2.11
Hues6-EB	 561.99	        2.11	        2.11
--------------------------------------------------------------

2. Examine the integrity of the RNA samples

  • 100ml agarose gel + 8ul SYBR safe

3. Dilute RNA samples in PCR 8-tube strip (-80C)

1) 1:10 2) 1:10 3) 1:4/1:6/1:5.6 --> 1ng/ul 4) 1:5 --> 0.2ng/ul 5) 1:10 --> 0.02ng/ul