Rui:LabNotes/SingleCell/2011-1-20: Difference between revisions
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=1. Measure the concentration of the RNA samples= | =1. Measure the concentration of the RNA samples= | ||
-------------------------------------------------------------- | -------------------------------------------------------------- | ||
RNA ID | RNA ID [ng/ul] 260/280 260/230 | ||
-------------------------------------------------------------- | -------------------------------------------------------------- | ||
Hues54 | Hues54 414.50 2.06 2.01 | ||
Hues6-ES 641.13 2.12 2.11 | Hues6-ES 641.13 2.12 2.11 | ||
Hues6-EB 561.99 2.11 2.11 | Hues6-EB 561.99 2.11 2.11 |
Revision as of 21:00, 20 January 2011
1. Measure the concentration of the RNA samples
-------------------------------------------------------------- RNA ID [ng/ul] 260/280 260/230 -------------------------------------------------------------- Hues54 414.50 2.06 2.01 Hues6-ES 641.13 2.12 2.11 Hues6-EB 561.99 2.11 2.11 --------------------------------------------------------------
2. Examine the integrity of the RNA samples
- 100ml agarose gel + 8ul SYBR safe
3. Dilute RNA samples in PCR 8-tube strip (-80C)
1) 1:10 2) 1:10 3) 1:4/1:6/1:5.6 --> 1ng/ul 4) 1:5 --> 0.2ng/ul 5) 1:10 --> 0.02ng/ul