Rui:LabNotes/SingleCell/2011-1-20: Difference between revisions

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=1. Measure the concentration of the RNA samples=
=1. Measure the concentration of the RNA samples=
  --------------------------------------------------------------
  --------------------------------------------------------------
  RNA ID [ng/ul] 260/280 260/230
  RNA ID         [ng/ul] 260/280 260/230
  --------------------------------------------------------------  
  --------------------------------------------------------------  
  Hues54 414.50         2.06         2.01
  Hues54         414.50         2.06         2.01
  Hues6-ES        641.13         2.12         2.11
  Hues6-ES        641.13         2.12         2.11
  Hues6-EB        561.99         2.11         2.11
  Hues6-EB        561.99         2.11         2.11

Revision as of 21:00, 20 January 2011

1. Measure the concentration of the RNA samples

--------------------------------------------------------------
RNA ID          [ng/ul]	260/280 	260/230
-------------------------------------------------------------- 
Hues54          414.50	        2.06	        2.01
Hues6-ES        641.13	        2.12	        2.11
Hues6-EB        561.99	        2.11	        2.11
--------------------------------------------------------------

2. Examine the integrity of the RNA samples

  • 100ml agarose gel + 8ul SYBR safe

3. Dilute RNA samples in PCR 8-tube strip (-80C)

1) 1:10 2) 1:10 3) 1:4/1:6/1:5.6 --> 1ng/ul 4) 1:5 --> 0.2ng/ul 5) 1:10 --> 0.02ng/ul