Rui:Haplotying: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Sam Chiang
>RuiLiu
Line 5: Line 5:


*Cell lysis buffer (1*)
*Cell lysis buffer (1*)
200mM NaCl
  20mM EDTA
  20mM EDTA
  10mM Tris-HCl (pH 8)
  10mM Tris-HCl (pH 8)
200mM NaCl
  0.2% TritonX-100
  0.2% TritonX-100
  (0.1 AU/ml Qiagen Proteinase 7.5AU)
  (0.1 AU/ml Qiagen Proteinase 7.5AU)

Revision as of 01:50, 3 February 2011

Background

Protocol

File:Design.ppt

  • Cell lysis buffer (1*)
200mM NaCl
20mM EDTA
10mM Tris-HCl (pH 8)
0.2% TritonX-100
(0.1 AU/ml Qiagen Proteinase 7.5AU)

Primers

File:Primers for Tn-based-haplotyping.xls

2011

<calendar> name=Rui:LabNotes/Haplotyping format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>