AlanFung:LabNotes/CTCF/2011-2-14: Difference between revisions

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*Nextera Sample Prep Kit
*Nextera Sample Prep Kit
*Input DNA
*Input DNA
*
*Bisulfite conversion kit
*Qiagen Purification Kit


==Protocol==
==Protocol==

Revision as of 01:19, 15 February 2011

Nextera Methyl-Seq DNA Sample Prep

Objective

  • Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100ng starting amount) and size around 200-1000BP

Samples & Materials

  • Nextera Sample Prep Kit
  • Input DNA
  • Bisulfite conversion kit
  • Qiagen Purification Kit

Protocol

  • Nuclease Free water
100ng input DNA 
4ul 5x Nextera HMW Reaction Buffer
1ul Nextera Methyl-Seq Tagmentation Mix