AlanFung:LabNotes/CTCF/2011-2-14: Difference between revisions

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>Alan6017518
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  4ul 5x Nextera HMW Reaction Buffer
  4ul 5x Nextera HMW Reaction Buffer
  1ul Nextera Methyl-Seq Tagmentation Mix
  1ul Nextera Methyl-Seq Tagmentation Mix
==Results==
==Suggestions==

Revision as of 01:29, 15 February 2011

Nextera Methyl-Seq DNA Sample Prep

Objective

  • Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100ng starting amount) and size around 200-1000BP

Samples & Materials

  • Nextera Sample Prep Kit_Epicentre_NMS072910B
  • Input DNA
  • Bisulfite conversion kit
  • Qiagen Purification Kit

Overview

  • Tagmentation
  • Purification
  • Fill-in
  • Purification
  • Bisulfite Conversion
  • Purification
  • bPCR-compatible sites and library enrichment

Protocol

  • Nuclease Free water
100ng input DNA 
4ul 5x Nextera HMW Reaction Buffer
1ul Nextera Methyl-Seq Tagmentation Mix

Results

Suggestions