AlanFung:LabNotes/CTCF/2011-2-28: Difference between revisions
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>Alan6017518 (Created page with '='''Nextera Methyl-Seq DNA Sample Prep on 100cells'''= ==Objective== *Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100 cells, ~600pg DNA s…') |
>Alan6017518 |
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*Bisulfite conversion kit | *Bisulfite conversion kit | ||
*Qiagen Purification Kit | *Qiagen Purification Kit | ||
==Overview== | |||
*Tagmentation | |||
*Purification | |||
*Fill-in | |||
*Purification | |||
*Bisulfite Conversion | |||
*Purification | |||
*bPCR-compatible sites and library enrichment |
Revision as of 19:09, 2 March 2011
Nextera Methyl-Seq DNA Sample Prep on 100cells
Objective
- Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100 cells, ~600pg DNA starting amount) with GM12878 gDNA.
Samples & Materials
- Nextera Sample Prep Kit_Epicentre_NMS072910B
NexteraTM Methyl-Seq Tagmentation Mix (Illumina-compatible) 5X Nextera Reaction Buffer (HMW) 50X Nextera Primer Cocktail (Illumina-compatible) 50X Nextera Adaptor 2 (Illumina-compatible) 2X Nextera Fill-in Buffer 2X Nextera PCR Buffer 200X Nextera Read 1 Primer 200X Nextera Read 2 Primer 200X Nextera Index Read Primer Nextera Methyl-Seq Polymerase
- Input DNA
- Bisulfite conversion kit
- Qiagen Purification Kit
Overview
- Tagmentation
- Purification
- Fill-in
- Purification
- Bisulfite Conversion
- Purification
- bPCR-compatible sites and library enrichment