AlanFung:LabNotes/CTCF/2011-2-28: Difference between revisions

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>Alan6017518
(Created page with '='''Nextera Methyl-Seq DNA Sample Prep on 100cells'''= ==Objective== *Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100 cells, ~600pg DNA s…')
 
>Alan6017518
Line 20: Line 20:
*Bisulfite conversion kit
*Bisulfite conversion kit
*Qiagen Purification Kit
*Qiagen Purification Kit
==Overview==
*Tagmentation
*Purification
*Fill-in
*Purification
*Bisulfite Conversion
*Purification
*bPCR-compatible sites and library enrichment

Revision as of 19:09, 2 March 2011

Nextera Methyl-Seq DNA Sample Prep on 100cells

Objective

  • Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100 cells, ~600pg DNA starting amount) with GM12878 gDNA.

Samples & Materials

  • Nextera Sample Prep Kit_Epicentre_NMS072910B
NexteraTM Methyl-Seq Tagmentation Mix (Illumina-compatible) 
5X Nextera Reaction Buffer (HMW)
50X Nextera Primer Cocktail (Illumina-compatible) 
50X Nextera Adaptor 2 (Illumina-compatible) 
2X Nextera Fill-in Buffer 
2X Nextera PCR Buffer 
200X Nextera Read 1 Primer 
200X Nextera Read 2 Primer 
200X Nextera Index Read Primer  
Nextera Methyl-Seq Polymerase 
  • Input DNA
  • Bisulfite conversion kit
  • Qiagen Purification Kit

Overview

  • Tagmentation
  • Purification
  • Fill-in
  • Purification
  • Bisulfite Conversion
  • Purification
  • bPCR-compatible sites and library enrichment