Calendar/2011-3-28: Difference between revisions
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>Alan6017518 (Created page with '==Library Construction on sample 1-21 from Dr. Heller== ===Qubit Quantification=== ===Tagmentation Reaction=== *Prior to assembling the reaction, briefly centrifuge the 5X Next…') |
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==Library Construction on sample 1-21 from Dr. Heller== | ==Library Construction on sample 1-21 from Dr. Heller== | ||
===Qubit Quantification=== | ===Qubit Quantification=== | ||
*dilute 25ul 200X dye with 4975ul buffer | |||
===Tagmentation Reaction=== | ===Tagmentation Reaction=== |
Revision as of 20:16, 28 March 2011
Library Construction on sample 1-21 from Dr. Heller
Qubit Quantification
- dilute 25ul 200X dye with 4975ul buffer
Tagmentation Reaction
- Prior to assembling the reaction, briefly centrifuge the 5X Nextera Reaction Buffer and Nextera Enzyme Mix tubes to assure that the reagents are at the bottom of the tubes.
- Assemble the following reaction components on ice, in the order listed:
x µl Nuclease-Free Water 50 ng Target DNA (in T10E1 Buffer [10 mM Tris-HCl (pH 7.5), 1 mM EDTA]) 4 µl 5X Nextera Reaction Buffer LMW or HMW (see Important Considerations, no. 1, p. 3) 1 µl Nextera Enzyme Mix (Illumina-compatible) 20 µl Total reaction volume