Calendar/2011-3-28: Difference between revisions
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>Alan6017518 |
>Alan6017518 |
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*dilute 25ul 200X dye with 4975ul buffer | *dilute 25ul 200X dye with 4975ul buffer | ||
{| {{table}} | {| {{table}} | ||
| Sample||Concentration | | Sample||Concentration(ng/ml)||Sample Concentration (ug/ml) | ||
|- | |- | ||
| |||| | | 1||37.2||7.44 | ||
|- | |- | ||
| | | 2||103||20.6 | ||
|- | |- | ||
| |||| | | 3||Out Of Range||5.4 | ||
|- | |- | ||
| | | 4||27||9.56 | ||
|- | |- | ||
| |||| | | 5||47.8||2.55 | ||
|- | |- | ||
| | | 6||12.7|| | ||
|- | |- | ||
| || | | 7||Out Of Range|| | ||
|- | |- | ||
| | | 8||Out Of Range|| | ||
|- | |- | ||
| || | | 9||Out Of Range|| | ||
|- | |- | ||
| | | 10||Out Of Range|| | ||
|- | |- | ||
| || | | 11||Out Of Range|| | ||
|- | |- | ||
| 12||Out Of Range|| | |||
|- | |- | ||
| || | | 13||Out Of Range|| | ||
|- | |- | ||
| | | 14||Out Of Range|| | ||
|- | |- | ||
| || | | 15||Out Of Range|| | ||
|- | |- | ||
| | | 16||Out Of Range|| | ||
|- | |- | ||
| || | | 17||Out Of Range|| | ||
|- | |- | ||
| | | 18||Out Of Range|| | ||
|- | |- | ||
| || | | 19||Out Of Range|| | ||
|- | |- | ||
| | | 20||Out Of Range|| | ||
|- | |- | ||
| 21||Out Of Range|| | |||
| 21||Out Of Range | |||
|} | |} | ||
Revision as of 21:12, 28 March 2011
Library Construction on sample 1-21 from Dr. Heller
Qubit Quantification
- dilute 25ul 200X dye with 4975ul buffer
Sample | Concentration(ng/ml) | Sample Concentration (ug/ml) |
1 | 37.2 | 7.44 |
2 | 103 | 20.6 |
3 | Out Of Range | 5.4 |
4 | 27 | 9.56 |
5 | 47.8 | 2.55 |
6 | 12.7 | |
7 | Out Of Range | |
8 | Out Of Range | |
9 | Out Of Range | |
10 | Out Of Range | |
11 | Out Of Range | |
12 | Out Of Range | |
13 | Out Of Range | |
14 | Out Of Range | |
15 | Out Of Range | |
16 | Out Of Range | |
17 | Out Of Range | |
18 | Out Of Range | |
19 | Out Of Range | |
20 | Out Of Range | |
21 | Out Of Range |
Tagmentation Reaction
- Prior to assembling the reaction, briefly centrifuge the 5X Nextera Reaction Buffer and Nextera Enzyme Mix tubes to assure that the reagents are at the bottom of the tubes.
- Assemble the following reaction components on ice, in the order listed:
x µl Nuclease-Free Water 50 ng Target DNA (in T10E1 Buffer [10 mM Tris-HCl (pH 7.5), 1 mM EDTA]) 4 µl 5X Nextera Reaction Buffer LMW or HMW (see Important Considerations, no. 1, p. 3) 1 µl Nextera Enzyme Mix (Illumina-compatible) 20 µl Total reaction volume