Calendar/2011-3-28: Difference between revisions
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>Alan6017518 |
>Alan6017518 |
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| 21||Out Of Range|| | | 21||Out Of Range|| | ||
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*Samples are too dilute | |||
===Tagmentation Reaction=== | ===Tagmentation Reaction=== |
Revision as of 21:12, 28 March 2011
Library Construction on sample 1-21 from Dr. Heller
Qubit Quantification
- dilute 25ul 200X dye with 4975ul buffer
Sample | Concentration(ng/ml) | Sample Concentration (ug/ml) |
1 | 37.2 | 7.44 |
2 | 103 | 20.6 |
3 | Out Of Range | 5.4 |
4 | 27 | 9.56 |
5 | 47.8 | 2.55 |
6 | 12.7 | |
7 | Out Of Range | |
8 | Out Of Range | |
9 | Out Of Range | |
10 | Out Of Range | |
11 | Out Of Range | |
12 | Out Of Range | |
13 | Out Of Range | |
14 | Out Of Range | |
15 | Out Of Range | |
16 | Out Of Range | |
17 | Out Of Range | |
18 | Out Of Range | |
19 | Out Of Range | |
20 | Out Of Range | |
21 | Out Of Range |
- Samples are too dilute
Tagmentation Reaction
- Prior to assembling the reaction, briefly centrifuge the 5X Nextera Reaction Buffer and Nextera Enzyme Mix tubes to assure that the reagents are at the bottom of the tubes.
- Assemble the following reaction components on ice, in the order listed:
x µl Nuclease-Free Water 50 ng Target DNA (in T10E1 Buffer [10 mM Tris-HCl (pH 7.5), 1 mM EDTA]) 4 µl 5X Nextera Reaction Buffer LMW or HMW (see Important Considerations, no. 1, p. 3) 1 µl Nextera Enzyme Mix (Illumina-compatible) 20 µl Total reaction volume