Sam:LabNotes/Micro-manipulation/2011-4-12: Difference between revisions
Jump to navigation
Jump to search
>Sam Chiang (Created page with '='''Single E.coli cell micromanipulation test on 04-12-'11'''= ==Testing objectives== *Focus on the performance of fluorescently stained cells. **Question 1: Can we see the same…') |
>Sam Chiang |
||
Line 19: | Line 19: | ||
==E.coli cell microscopy analysis== | ==E.coli cell microscopy analysis== | ||
[[File:dapi_stained_dish-bf1.png|400px]] | |||
*Next: | *Next: |
Revision as of 22:49, 12 April 2011
Single E.coli cell micromanipulation test on 04-12-'11
Testing objectives
- Focus on the performance of fluorescently stained cells.
- Question 1: Can we see the same number of samples under the BF GFP/DAPI filters? (If not, what do we missed, E.coli or unknown particles?)
- Question 2: Do we observe flourescently stained particles in negative control (blank slide)
Cell resource
- Using old stored E.coli K-12 culture (4C, 1XPBS, March, 2010)
- Estimated concentration = 10^9 CFU/mL
E.coli stain (SYBR-safe / DAPI)
- Prepare dye working solution (10X)
- Add 1uL SYBR-safe(10,000X) with 50uL 1X TE in a 1.7mL tube wrapped in foil => 200X
- Add 10uL SYBR-safe(200X) with 190uL 1X TE in a 1.7mL Amber tube => 10X
- Transfer 2uL E.coli culture into the 200uL dye working solution
- Same method was prepared for DAPI staining.
E.coli cell microscopy analysis
File:Dapi stained dish-bf1.png
- Next: