Sam:LabNotes/Microbiome-new/2011-4-27: Difference between revisions
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>Sam Chiang |
>Sam Chiang |
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**http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-14 | **http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-14 | ||
*2nd size-selection | *2nd size-selection | ||
http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-15 | **http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-15 |
Revision as of 00:46, 28 April 2011
Mouse gut bacteria SAG ("c5")preparation
Objective
- I prepared two Illumina libraries using limited MDA (2hr incubation) strategy, but only one of the samples was sequenced.
Illumina library amplification references
- Ligation product amplification with N2-indexed primer
- AMpure bead purification
- 2nd size-selection