Sam:LabNotes/Microbiome-new/2011-4-27: Difference between revisions
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>Sam Chiang |
>Sam Chiang |
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==Objective== | ==Objective== | ||
*I prepared two Illumina libraries using limited MDA (2hr incubation) strategy, but only one of the samples was sequenced. | *I prepared two Illumina libraries using limited MDA (2hr incubation) strategy, but only one of the samples was sequenced. | ||
** | **http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-3-2 | ||
*I still have the ligation product left from this sample. I will amplify it using N2-index primer and give it to Alan for sequencing. | |||
==Illumina library amplification references== | ==Illumina library amplification references== |
Revision as of 00:49, 28 April 2011
Mouse gut bacteria SAG ("c5")preparation
Objective
- I prepared two Illumina libraries using limited MDA (2hr incubation) strategy, but only one of the samples was sequenced.
- I still have the ligation product left from this sample. I will amplify it using N2-index primer and give it to Alan for sequencing.
Illumina library amplification references
- Ligation product amplification with N2-indexed primer
- AMpure bead purification
- 2nd size-selection