Rui:LabNotes/Collaborations/2011-5-20: Difference between revisions

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>RuiLiu
(Created page with ' ==Additional 10 cycles of direct lysis samples== * Continue with lab note on 5.19.11 * Additional 10 cycles for direct lysis samples (sperm and ES) in parallel with 500pg RNA a…')
 
>RuiLiu
Line 15: Line 15:
* 2nd strand with P8
* 2nd strand with P8
* PCR with 30 cycles
* PCR with 30 cycles
===QPCR===
[[File:5.20.11_remaining samples.jpg]]

Revision as of 18:18, 17 June 2011


Additional 10 cycles of direct lysis samples

  • Continue with lab note on 5.19.11
  • Additional 10 cycles for direct lysis samples (sperm and ES) in parallel with 500pg RNA and 150pg gDNA

QPCR curve

File:Additional 10 cycles 5.20.11.jpg

TBE PAGE

File:10 cycles 5.20.11.jpg


Repeat 2nd strands and PCR with the remaining samples

  • Continue with samples on 5.19.11, the remaining 9ul A-tailing product
  • 2nd strand with P8
  • PCR with 30 cycles

QPCR

File:5.20.11 remaining samples.jpg