Rui:LabNotes/Collaborations/2011-5-20: Difference between revisions
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>RuiLiu (Created page with ' ==Additional 10 cycles of direct lysis samples== * Continue with lab note on 5.19.11 * Additional 10 cycles for direct lysis samples (sperm and ES) in parallel with 500pg RNA a…') |
>RuiLiu |
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* 2nd strand with P8 | * 2nd strand with P8 | ||
* PCR with 30 cycles | * PCR with 30 cycles | ||
===QPCR=== | |||
[[File:5.20.11_remaining samples.jpg]] |
Revision as of 18:18, 17 June 2011
Additional 10 cycles of direct lysis samples
- Continue with lab note on 5.19.11
- Additional 10 cycles for direct lysis samples (sperm and ES) in parallel with 500pg RNA and 150pg gDNA
QPCR curve
File:Additional 10 cycles 5.20.11.jpg
TBE PAGE
Repeat 2nd strands and PCR with the remaining samples
- Continue with samples on 5.19.11, the remaining 9ul A-tailing product
- 2nd strand with P8
- PCR with 30 cycles