Rui:LabNotes/SingleCell/2011-7-1: Difference between revisions

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>Sam Chiang
m (Created page with '==Purification of HRCA results== ===MinElute=== NC, PC, 60pg gDNA, 1pg lysate 1, elute in 10ul, 1ul for TBE PAGE File:MinElute.jpg')
 
>RuiLiu
Line 4: Line 4:
NC, PC, 60pg gDNA, 1pg lysate 1, elute in 10ul, 1ul for TBE PAGE
NC, PC, 60pg gDNA, 1pg lysate 1, elute in 10ul, 1ul for TBE PAGE


[[File:MinElute.jpg]]
[[File:7.1.11_MinElute.jpg]]
 
 
===EtOH precipitation (ExoI treatment)===
 
PC, 1pg lysate 2 (treated with ExoI for 30min)
 
PC, 1pg lysate 3 (no treatment)
 
EtOH precipitation: 5ul NaOAc, 125ul EtOH, -80C 30min, spin 30min, huge pellet, resuspend in 30ul
 
[[File:7.6.11_quant.jpg]]
 
[[File:7.6.11_over.jpg]]

Revision as of 18:16, 7 July 2011

Purification of HRCA results

MinElute

NC, PC, 60pg gDNA, 1pg lysate 1, elute in 10ul, 1ul for TBE PAGE

File:7.1.11 MinElute.jpg


EtOH precipitation (ExoI treatment)

PC, 1pg lysate 2 (treated with ExoI for 30min)

PC, 1pg lysate 3 (no treatment)

EtOH precipitation: 5ul NaOAc, 125ul EtOH, -80C 30min, spin 30min, huge pellet, resuspend in 30ul

File:7.6.11 quant.jpg

File:7.6.11 over.jpg