Rui:LabNotes/SingleCell/2011-7-1: Difference between revisions
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>Sam Chiang m (Created page with '==Purification of HRCA results== ===MinElute=== NC, PC, 60pg gDNA, 1pg lysate 1, elute in 10ul, 1ul for TBE PAGE File:MinElute.jpg') |
>RuiLiu |
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NC, PC, 60pg gDNA, 1pg lysate 1, elute in 10ul, 1ul for TBE PAGE | NC, PC, 60pg gDNA, 1pg lysate 1, elute in 10ul, 1ul for TBE PAGE | ||
[[File: | [[File:7.1.11_MinElute.jpg]] | ||
===EtOH precipitation (ExoI treatment)=== | |||
PC, 1pg lysate 2 (treated with ExoI for 30min) | |||
PC, 1pg lysate 3 (no treatment) | |||
EtOH precipitation: 5ul NaOAc, 125ul EtOH, -80C 30min, spin 30min, huge pellet, resuspend in 30ul | |||
[[File:7.6.11_quant.jpg]] | |||
[[File:7.6.11_over.jpg]] |
Revision as of 18:16, 7 July 2011
Purification of HRCA results
MinElute
NC, PC, 60pg gDNA, 1pg lysate 1, elute in 10ul, 1ul for TBE PAGE
EtOH precipitation (ExoI treatment)
PC, 1pg lysate 2 (treated with ExoI for 30min)
PC, 1pg lysate 3 (no treatment)
EtOH precipitation: 5ul NaOAc, 125ul EtOH, -80C 30min, spin 30min, huge pellet, resuspend in 30ul