Brandon Sos: Difference between revisions
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[[Brandon:Protocols/tn5_production | tn5 production protocol]] | [[Brandon:Protocols/tn5_production | tn5 production protocol]] | ||
[[Brandon:Protocols/ | [[Brandon:Protocols/tn5_production_updated_7_30_2014 | tn5 production protocol updated (7/30/2014)]] | ||
Revision as of 23:48, 30 July 2014
Current Projects
Protocols
AMPure XP beads purification only (for >= 5 ng)
AMPure XP beads purification 2-step PCR (for <=5 ng)
Invitrogen MAXIscript T7 In vitro transcription kit
SMART MMLV RT protocol (for first strand synthesis)
standard PCR cycling for for different enzymes etc
enzyme reaction buffer compositions
tn5 production protocol updated (7/30/2014)
- notes
- minielute colums for <10 ul samples, otherwise use qiaquick columns
- N2 adaptor is illuminia adaptor. Nextera adaptor is nextera adaptor
Main Calendar
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2014/02/01 view=twoyear </calendar>
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2013/02/01 view=twoyear </calendar>
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2012/02/01 view=twoyear </calendar>
<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2011/02/01 view=twoyear </calendar>