Noi/NOTES/2011-9-13: Difference between revisions

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= Sanger sequencing results of the amplicons amplified by randomly tagging primers with and without USER =
= Sanger sequencing results of the amplicons amplified by randomly tagging primers with and without USER =
* 10 clones of positive control with no USER, it showed
* 10 clones of
** 8 clones showed very clear poly T sequences (TTTTTTT read by reverse primer, Syb_RP7). One clone show only 6 T and another clone showed TTTCTTT. I am not sure if the last one is the random sequence from the survival AmpFNU6.3Sol primer or the artifact of sequencing since the DNA chromatogram showed a very clear C peak. However based on the comment of the this sample result, it said that there was a spectral puul-up.
[[File:NP-NA-U-01.png| 400px]]<br>
[[File:NP-NA-U-09.png| 400px]]<br>
* 10 clones of positive control with no USER
** 4 clones with poly T
** 6 clones with random sequences (NNNNNNN)

Revision as of 23:53, 13 September 2011

Sanger sequencing results of the amplicons amplified by randomly tagging primers with and without USER

  • 10 clones of
    • 8 clones showed very clear poly T sequences (TTTTTTT read by reverse primer, Syb_RP7). One clone show only 6 T and another clone showed TTTCTTT. I am not sure if the last one is the random sequence from the survival AmpFNU6.3Sol primer or the artifact of sequencing since the DNA chromatogram showed a very clear C peak. However based on the comment of the this sample result, it said that there was a spectral puul-up.

File:NP-NA-U-01.png
File:NP-NA-U-09.png

  • 10 clones of positive control with no USER
    • 4 clones with poly T
    • 6 clones with random sequences (NNNNNNN)