Rui:SingelCell RNAseq: Difference between revisions

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>Jdeng
>RuiLiu
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*[[Rui:IVT note|IVT note]]
*[[Rui:IVT note|IVT note]]
*[[Rui:HRCA note|HRCA note]]
*[[Rui:HRCA note|HRCA note]]
==2013==
<calendar>
name=Rui:LabNotes/SingleCell
format=%name/%year-%month-%day
date=2013/10/1
view=oneyear
</calendar>


==2012==
==2012==
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view=oneyear
view=oneyear
</calendar>
</calendar>


==2011==
==2011==

Revision as of 23:53, 2 January 2013

Background

  • Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
  • Review papers:
  • Key papers:
  1. Single cell and cell lysis
  2. 1st cDNA synthesis and free primer removal
  3. 2nd cDNA synthesis: A-tailing vs random
  4. Amplification: PCR or IVT
  5. QC and clone

Protocols

QuickNotes

2013

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2013/10/1 view=oneyear </calendar>

2012

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>

2011

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>