AlanFung:LabNotes/Methylome/2012-1-17: Difference between revisions

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>Alan6017518
>Alan6017518
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*Covaris shearing of 5ug gDNA to ~400bp
*Covaris shearing of 5ug gDNA to ~400bp
*Contacted Yang at Kelly Frazer's lab to help me with it
*Contacted Yang at Kelly Frazer's lab to help me with it
==End repair & A-tailing==
==ligation to methylated Y adaptor==
==Bisulfite Conversion==
==PCR==
==Size selection==

Revision as of 22:30, 17 January 2012

GM12878 Library

Sample Preparation

  • Covaris shearing of 5ug gDNA to ~400bp
  • Contacted Yang at Kelly Frazer's lab to help me with it

End repair & A-tailing

ligation to methylated Y adaptor

Bisulfite Conversion

PCR

Size selection