AlanFung:LabNotes/Methylome/2012-1-17: Difference between revisions
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*Get GM12878 samples from Noi | *Get GM12878 samples from Noi | ||
*Measure concentration with qubit | *Measure concentration with qubit | ||
*Take 5ug of samples and prepare for shearing | *Take 5ug of samples and prepare for shearing (Make total volume to 100ul with ddh2o) | ||
*Covaris shearing of 5ug gDNA to ~400bp | *Covaris shearing of 5ug gDNA to ~400bp | ||
*Contacted Yang at Kelly Frazer's lab to help me with it | *Contacted Yang at Kelly Frazer's lab to help me with it |
Revision as of 22:42, 17 January 2012
GM12878 Library
Sample Preparation
- Get GM12878 samples from Noi
- Measure concentration with qubit
- Take 5ug of samples and prepare for shearing (Make total volume to 100ul with ddh2o)
- Covaris shearing of 5ug gDNA to ~400bp
- Contacted Yang at Kelly Frazer's lab to help me with it