AlanFung:LabNotes/Methylome/2012-1-17: Difference between revisions

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>Alan6017518
>Alan6017518
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*Get GM12878 samples from Noi
*Get GM12878 samples from Noi
*Measure concentration with qubit  
*Measure concentration with qubit  
*Take 5ug of samples and prepare for shearing (Make total volume to 100ul with ddh2o)
*Take 5ug of samples and prepare for shearing (Make total volume to 100ul with ddh2o)  
*Covaris shearing of 5ug gDNA to ~400bp
*Covaris shearing of 5ug gDNA to ~400bp
*Contacted Yang at Kelly Frazer's lab to help me with it
*Contacted Yang at Kelly Frazer's lab to help me with it

Revision as of 22:50, 17 January 2012

GM12878 Library

Sample Preparation

  • Get GM12878 samples from Noi
  • Measure concentration with qubit
  • Take 5ug of samples and prepare for shearing (Make total volume to 100ul with ddh2o)
  • Covaris shearing of 5ug gDNA to ~400bp
  • Contacted Yang at Kelly Frazer's lab to help me with it

End repair & A-tailing

ligation to methylated Y adaptor

Bisulfite Conversion

PCR

Size selection