AlanFung:LabNotes/Methylome/2012-1-17: Difference between revisions
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==End repair & A-tailing== | ==End repair & A-tailing== | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Content''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume''' | |||
|- | |||
| Fragmented DNA||variable | |||
|- | |||
| End Reapir buffer||10 | |||
|- | |||
| End Repair Enzyme Mix||5 | |||
|- | |||
| ddH2O||Variable | |||
|- | |||
| Volume||100 | |||
|} | |||
==ligation to methylated Y adaptor== | ==ligation to methylated Y adaptor== |
Revision as of 22:57, 17 January 2012
GM12878 Library
Sample Preparation
- Get GM12878 samples from Noi
- Measure concentration with qubit
- Take 5ug of samples and prepare for shearing (Make total volume to 100ul with ddh2o)
- Covaris shearing of 5ug gDNA to ~400bp
- Contacted Yang at Kelly Frazer's lab to help me with it
End repair & A-tailing
Content | Volume |
Fragmented DNA | variable |
End Reapir buffer | 10 |
End Repair Enzyme Mix | 5 |
ddH2O | Variable |
Volume | 100 |