Noi/NOTES/2012-5-16: Difference between revisions
Jump to navigation
Jump to search
>Noi (Created page with "* http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes '''Link to calendar''' == Probe production for the HiResChrPaint project == * Continued from: http://...") |
>Noi No edit summary |
||
Line 3: | Line 3: | ||
* Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-5-14 | * Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-5-14 | ||
* Since Dr. Zhang suggested that about 20% of the oligos having AP2 part should be fine. I will continue to digest the rest of amplified amplicons with DpnII and column purify | * Since Dr. Zhang suggested that about 20% of the oligos having AP2 part should be fine. I will continue to digest the rest of amplified amplicons with DpnII and column purify | ||
Probe IDs Conc. (ng/ul) Yields in 120ul (ug) Left over after trial (ug) | |||
#1-V4 83.70 10.04 5.00 | |||
#2-V4 125.10 15.01 12.00 | |||
#3-V4 110.10 13.21 13.20 | |||
#4-V4 133.80 16.06 8.00 | |||
#1-V6 122.50 14.70 14.70 | |||
#2-V6 106.20 12.74 12.74 | |||
#3-V6 132.20 15.86 15.86 | |||
#4-V6 101.70 12.20 12.20 |
Revision as of 20:21, 16 May 2012
Probe production for the HiResChrPaint project
- Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-5-14
- Since Dr. Zhang suggested that about 20% of the oligos having AP2 part should be fine. I will continue to digest the rest of amplified amplicons with DpnII and column purify
Probe IDs Conc. (ng/ul) Yields in 120ul (ug) Left over after trial (ug) #1-V4 83.70 10.04 5.00 #2-V4 125.10 15.01 12.00 #3-V4 110.10 13.21 13.20 #4-V4 133.80 16.06 8.00 #1-V6 122.50 14.70 14.70 #2-V6 106.20 12.74 12.74 #3-V6 132.20 15.86 15.86 #4-V6 101.70 12.20 12.20