Noi/NOTES/2012-5-16: Difference between revisions

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>Noi
(Created page with "* http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes '''Link to calendar''' == Probe production for the HiResChrPaint project == * Continued from: http://...")
 
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* Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-5-14
* Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-5-14
* Since Dr. Zhang suggested that about 20% of the oligos having AP2 part should be fine. I will continue to digest the rest of amplified amplicons with DpnII and column purify
* Since Dr. Zhang suggested that about 20% of the oligos having AP2 part should be fine. I will continue to digest the rest of amplified amplicons with DpnII and column purify
  Probe IDs      Conc. (ng/ul)  Yields in 120ul (ug) Left over after trial (ug)
#1-V4           83.70         10.04                   5.00
#2-V4         125.10         15.01                 12.00
#3-V4         110.10         13.21                 13.20
#4-V4         133.80         16.06                 8.00
#1-V6         122.50         14.70                 14.70
#2-V6         106.20          12.74                 12.74
#3-V6         132.20         15.86                 15.86
#4-V6         101.70         12.20                 12.20

Revision as of 20:21, 16 May 2012

Probe production for the HiResChrPaint project

 Probe IDs       	Conc. (ng/ul)  	 Yields in 120ul (ug)	Left over after trial (ug)
#1-V4	          83.70	         10.04	                  5.00
#2-V4	        125.10	         15.01	                12.00
#3-V4	        110.10	         13.21	                13.20
#4-V4	        133.80	         16.06	                 8.00
#1-V6	        122.50	         14.70	                14.70
#2-V6	        106.20           12.74	                12.74
#3-V6	        132.20	         15.86	                15.86
#4-V6	        101.70	         12.20	                12.20