Tina:MESC rainbow line: Difference between revisions
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>TinaLo (Created page with "==mESC rainbow line expansion== *'''5/2''' **Received one vial of cells from Inna, and stored it in the N2(l) tank located at CERC. *'''5/3 discussed with Dr. Blue Lake in D...") |
>TinaLo |
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*'''5/21 Passaging (Blue and Tina)''' | *'''5/21 Passaging (Blue and Tina)''' | ||
**1:3 wells look very healthy; 1:6 wells look good, and 1:12 well can wait for one more day | **1:3 wells look very healthy; 1:6 wells look good, and 1:12 well can wait for one more day. | ||
**Passage one well of 1:3 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well) | **Passage one well of 1:3 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well) | ||
**Passage one well of 1:6 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well) | **Passage one well of 1:6 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well) | ||
**Frozen down 8 vials of P5 InsACGGsAlov3 (from one 1:3 well and one 1:6 well) | **Frozen down 8 vials of P5 InsACGGsAlov3 (from one 1:3 well and one 1:6 well) | ||
*'''5/22''' | |||
**P6 cells look nice. Cells were passaged well yesterday. | |||
**P5 1:12 well shows a few differentiated colonies, and therefore is discarded to maintain good cell quality. |
Revision as of 17:32, 22 May 2012
mESC rainbow line expansion
- 5/2
- Received one vial of cells from Inna, and stored it in the N2(l) tank located at CERC.
- 5/3 discussed with Dr. Blue Lake in Dr. Yang Xu’s lab
- 5/7 preparing MEF with Blue
- 5/8 thawing
- Thaw cells in one 6-well plate
- 5/11
- some colonies appear to show
- 5/14 Passaging
- The culture looks ok, but there is some differentiation… so Blue passaged at several different dilutions (the lower ones to try and get rid of the differentiated cells).
- Passage one 6 well into one 1:3, one 1:4, one 1:5, and one 1:6 well.
- 5/18 Passaging
- Passage the 1:3 well into two 1:3 wells, two 1:6 wells, and one 1:12 well.
- Frozen down 3 vials of P4 InsACGGsAlov3 (from one 1:4 well, one 1:5 well, and one 1:6 well).
- 5/21 Passaging (Blue and Tina)
- 1:3 wells look very healthy; 1:6 wells look good, and 1:12 well can wait for one more day.
- Passage one well of 1:3 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well)
- Passage one well of 1:6 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well)
- Frozen down 8 vials of P5 InsACGGsAlov3 (from one 1:3 well and one 1:6 well)
- 5/22
- P6 cells look nice. Cells were passaged well yesterday.
- P5 1:12 well shows a few differentiated colonies, and therefore is discarded to maintain good cell quality.