Matthew Cai: Difference between revisions

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=====Ampligase Efficiency Test=====
=====Ampligase Efficiency Test=====
*[[Matt:LabNotes/2014-4-9|Ampligase Test First Try]]
*[[Matt:LabNotes/2014-4-15|Ampligase Test Second Try]]
*[[Matt:LabNotes/2014-5-16|Detecting ppMALAT1 Hybridization]]
*[[Matt:LabNotes/2014-5-16|Detecting ppMALAT1 Hybridization]]
*[[Matt:LabNotes/2014-5-27#Exo_I.2FIII_Test|Testing Exo I/III Digestion of Hybridized Padlock Probes]]
*[[Matt:LabNotes/2014-5-27#Exo_I.2FIII_Test|Testing Exo I/III Digestion of Hybridized Padlock Probes]]

Revision as of 01:55, 5 June 2014

Notebook

2014

<calendar> name=Matt format=%name:LabNotes/%year-%month-%day date=2014/01/01 view=oneyear </calendar>

2013

<calendar> name=Matt format=%name:LabNotes/%year-%month-%day date=2013/01/01 view=oneyear </calendar>

Projects

in Situ Sequencing

Probe/Primer Design

Probe Production and Testing

Rolony Experiments

Ampligase Efficiency Test

Decoding

Protocols

Sequencing Runs

130325_MiSeq: CA12k capture of gDNA, cDNAwRNase, and cDNA-RNase
130524_HL152 (Lane 2, unassigned): CA12k oligos
130628_HL155 (Lane 3, Indx 10 & 12): Agi26k_0gap and Agi26k_20gap oligos
130729_MiSeq: Agi26k_0gap and Agi26k_20gap capture of gDNA and cDNA

Probe Sets

CA12k: 12,355 probes (170nt)
Agi26k_0gap: 12,964 probes (193nt)
Agi26k_20gap: 13,179 probes (193nt)