Tina:LabNotes/2012-5-25: Difference between revisions
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>TinaLo (Created page with "**Passage one well of 1:12 P6 InsACGGsAlov3 into 1:6 (1 well), 1:12 (1 well) and 1:24 (1 well) **Frozen down 3 vials of P6 InsACGGsAlov3 (from one 1:12 well and the remaining ...") |
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**Passage one well of 1:12 P6 InsACGGsAlov3 into 1:6 (1 well), 1:12 (1 well) and 1:24 (1 well) | **Passage one well of 1:12 P6 InsACGGsAlov3 into 1:6 (1 well), 1:12 (1 well) and 1:24 (1 well) | ||
**Frozen down 3 vials of P6 InsACGGsAlov3 (from one 1:12 well and the remaining part of the passaged 1:12 well) | **Frozen down 3 vials of P6 InsACGGsAlov3 (from one 1:12 well and the remaining part of the passaged 1:12 well). Put cryovials at -80. | ||
**[[Preparation of 0.1% gelatin solution]] | **[[Preparation of 0.1% gelatin solution]] | ||
**[[Preparation of gelatin coated plates]] | **[[Preparation of gelatin coated plates]] | ||
**Seed MEF on gelatin coating plates (using [[MEF culture medium]]) | **Seed MEF on gelatin coating plates (using [[MEF culture medium]]) |
Revision as of 22:54, 4 June 2012
- Passage one well of 1:12 P6 InsACGGsAlov3 into 1:6 (1 well), 1:12 (1 well) and 1:24 (1 well)
- Frozen down 3 vials of P6 InsACGGsAlov3 (from one 1:12 well and the remaining part of the passaged 1:12 well). Put cryovials at -80.
- Preparation of 0.1% gelatin solution
- Preparation of gelatin coated plates
- Seed MEF on gelatin coating plates (using MEF culture medium)