Andrew Richards: Difference between revisions
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[http://genome-tech.ucsd.edu/LabNotes/index.php/Brandon:LabNotes/Project1/2014-11-18 Brandon's protocol for DNA purification after guanidine HCl] | [http://genome-tech.ucsd.edu/LabNotes/index.php/Brandon:LabNotes/Project1/2014-11-18 Brandon's protocol for DNA purification after guanidine HCl] | ||
[[Arichard:Protocols/tn5059|Tn5059 protocol (supermutant)]] | |||
==Computational stuff== | ==Computational stuff== |
Revision as of 19:08, 25 February 2015
Notebook
MagVigen DNA Select Focus 300/600 beads, October 1, 2014
Bulk Nextera libraries
January 25, 2015 (SW480 and lymphocyte)
February 10, 2015 (1-02 and 25-01)
MIDAS
C1 DNA Experiments
C1 DNA 5-10 um Chip on July 30, 2014
Misc
Tn5059 (super mutant) test, February 17, 2015
Protocols
Glass micropipette fabrication
MDA on microarray as of September 5, 2013
MDA amplicon extraction and processing
MDA amplicon extraction and processing as of September 5, 2013
MDA amplicon tagmentation and PCR
MDA amplicon tagmentation and PCR as of September 5, 2013
Technics PEIIB Planar Etcher for PDMS surface activation
Neuronal nuclei isolation as of December 3, 2013
Brandon's protocol for DNA purification after guanidine HCl
Computational stuff
Email from Jeff with tips on CNV calling
Sorts
2014 May 20 sort for 25-00 AD and 7-03 Control
2014 October 09 sort for 1-02 AD and 25-01 Control
2014 October 21 sort for SW480 and lymphocytes
Samples
Sequenced libraries for CNV analysis
- This is old. See Samples for full information
- This is also old. See Sequenced libraries
Reagents
High molecular weight (HMW) buffer recipe
- From Rui's wiki
Hypotonic lysis solution (HLS)
Nextera XT v2 primers (8 x 12)
Slides
Progress report for February 26, 2013
Progress report for February 26, 2013 REVISED
Progress report for April 25, 2013
Progress report for June 5, 2013
Progress report for September 04, 2013