Arichard:Samples/MDA microarray samples: Difference between revisions
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>Andrew No edit summary |
>Andrew No edit summary |
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==MDA microarray== | ==MDA microarray== | ||
===September 13=== | |||
* All nuclei from Alzheimer's patient: CTX+ (Neuronal cortex), CTX- (Non-neuronal cortex), CBL+ (Neuronal cerebellum), and CBL- (Neuronal cerebellum). | * All nuclei from Alzheimer's patient: CTX+ (Neuronal cortex), CTX- (Non-neuronal cortex), CBL+ (Neuronal cerebellum), and CBL- (Neuronal cerebellum). | ||
** 2401 CTX+ needs no dilution. | ** 2401 CTX+ needs no dilution. | ||
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** Control brain nuclei: 60831 FITC+ (Neuronal) and 60831 FITC- (Non-neuronal). These samples need to be diluted 1:10 in PBS (e.g., 5 ul:45 ul). | ** Control brain nuclei: 60831 FITC+ (Neuronal) and 60831 FITC- (Non-neuronal). These samples need to be diluted 1:10 in PBS (e.g., 5 ul:45 ul). | ||
** Cell-free, metaphase chromosomes: GM20431 at 150 parts/ul. Optimal dilution for this sample has not been determined, so we are currently doing both 1:1 (meaning no dilution) and 1:5, both 50 ul total volume. | ** Cell-free, metaphase chromosomes: GM20431 at 150 parts/ul. Optimal dilution for this sample has not been determined, so we are currently doing both 1:1 (meaning no dilution) and 1:5, both 50 ul total volume. | ||
* Eukaryotic samples are stored at 4deg C. Prokaryotic samples are stored at -20deg C as glycerol stocks. | |||
===October 10=== | |||
* Below is the current list of all the samples and required dilutions. | |||
{| border="1" | |||
|+ Samples and dilutions | |||
| Skn || 1:1 | | |||
|- | |||
| Sea || 1:1 | | |||
|- | |||
| CtlCtx+ || 1:10 | | |||
|- | |||
| CtlCtx- || 1:10 | | |||
|- | |||
| AlzCtx+ || 1:1 | | |||
|- | |||
|AlzCtx- || 1:10 | | |||
|- | |||
|AlzCbl+ || 1:20 | | |||
|- | |||
|AlzCbl- || 1:10 | | |||
|- | |||
| DowCtx+ || 1:10 | | |||
|- | |||
| DowCtx || 1:20 | | |||
|} |
Revision as of 20:25, 10 October 2012
MDA microarray
September 13
- All nuclei from Alzheimer's patient: CTX+ (Neuronal cortex), CTX- (Non-neuronal cortex), CBL+ (Neuronal cerebellum), and CBL- (Neuronal cerebellum).
- 2401 CTX+ needs no dilution.
- 2401 CBL+ needs 1:10 dilution.
- Jeff didn't say what dilutions are needed for 2401 CTX- and 2401 CBL-.
- Other samples are as follows
- Two types of sea bacteria: Synechococcus and prochlorococcus. These samples are already at the right concentrations, 10-20 parts/ul.
- Skin bacteria sample from microbiome project. Heterogeneous. I need to ask Jeff about these dilutions.
- Control brain nuclei: 60831 FITC+ (Neuronal) and 60831 FITC- (Non-neuronal). These samples need to be diluted 1:10 in PBS (e.g., 5 ul:45 ul).
- Cell-free, metaphase chromosomes: GM20431 at 150 parts/ul. Optimal dilution for this sample has not been determined, so we are currently doing both 1:1 (meaning no dilution) and 1:5, both 50 ul total volume.
- Eukaryotic samples are stored at 4deg C. Prokaryotic samples are stored at -20deg C as glycerol stocks.
October 10
- Below is the current list of all the samples and required dilutions.
Skn | |
Sea | |
CtlCtx+ | |
CtlCtx- | |
AlzCtx+ | |
AlzCtx- | |
AlzCbl+ | |
AlzCbl- | |
DowCtx+ | |
DowCtx |